Identification of many microRNAs that copurify with polyribosomes in mammalian neurons
Identification of many microRNAs that copurify with polyribosomes in mammalian neurons
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DOI：
10.1073/pnas.2333854100
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发表时间：
2004-01-06
影响因子：
11.1
通讯作者：
Ruvkun, G
中科院分区：
文献类型：
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作者：
Kim, J;Krichevsky, A;Ruvkun, G
哺乳动物树突中的局部翻译可能在突触可塑性中发挥作用，并有助于学习和记忆的分子基础。控制神经元局部翻译的调控机制尚不清楚。我们提出了microRNAs （miRNAs）的作用，一类非编码rna，作为神经元翻译调节的介质。我们已经鉴定出86种mirna在哺乳动物神经元中表达，其中40种以前没有报道过。这些mirna的一个子集在皮层培养物中表现出暂时调节的表达。此外，所有被测试的mirna都与多核糖体（活性翻译位点）发生缩合。这些发现表明，在哺乳动物神经元中，大量不同的mirna可能起调节翻译的作用。
Localized translation in mammalian dendrites may play a role in synaptic plasticity and contribute to the molecular basis for learning and memory. The regulatory mechanisms that control localized translation in neurons are not well understood. We propose a role for microRNAs (miRNAs), a class of noncoding RNAs, as mediators of neuronal translational regulation. We have identified 86 miRNAs expressed in mammalian neurons, of which 40 have not previously been reported. A subset of these miRNAs exhibits temporally regulated expression in cortical cultures. Moreover, all of the miRNAs that were tested cofractionate with polyribosomes, the sites of active translation. These findings indicate that a large, diverse population of miRNAs may function to regulate translation in mammalian neurons.