Fructo-oligosaccharide-Induced Transient Increases in Cecal Immunoglobulin A Concentrations in Rats Are Associated with Mucosal Inflammation in Response to Increased Gut Permeability
Fructo-oligosaccharide-Induced Transient Increases in Cecal Immunoglobulin A Concentrations in Rats Are Associated with Mucosal Inflammation in Response to Increased Gut Permeability
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DOI：
10.3945/jn.117.253955
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发表时间：
2017-10-01
影响因子：
4.2
通讯作者：
Morita, Tatsuya
中科院分区：
文献类型：
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作者：
Genda, Tomomi;Sasaki, Yuta;Morita, Tatsuya
背景资料：喂食低聚果糖（FOS）的大鼠盲肠内容物中免疫球蛋白（IG）A浓度瞬时升高的机制尚不清楚。目的：本研究旨在测试伊加浓度升高是否代表FOS诱导的盲肠通透性增加的炎症反应的一个方面。7周龄雄性Wistar大鼠饲喂不含纤维的半纯化饲料（FFP），添加或不添加FOS（60 g/kg饲料），连续9或58 d [实验（expt.）1]，7 d（expt. 2），或7或56 d（expt. 3）。除了测量盲肠内容物中的伊加浓度外，我们还评估了肠道通透性、炎症反应（实验）。1），盲肠固有层中伊加浆细胞的数量，盲肠粘膜中多聚IG受体（pIgR）的表达（实验1）。2），和盲肠粘液层的条件（实验。3）.结果：与FFP组相比，FOS组大鼠盲肠伊加浓度升高15倍（P < 0.05）。肠通透性估计尿铬EDTA排泄，细菌易位到肠系膜淋巴结，髓过氧化物酶活性，盲肠粘膜中的炎症细胞因子基因的表达是在福斯喂养大鼠比在大鼠喂养FFP 9天。在喂食FOS 58 d的大鼠中未观察到这些效应（实验1）。1）。与FFP组相比，FOS组大鼠盲肠伊加浓度升高，盲肠粘膜中伊加浆细胞数和pIgR蛋白表达增加（实验组）。2）。盲肠切片中的阿辛蓝染色表明，喂食FOS 7 d的大鼠上皮表面的粘液层明显破坏，但喂食FOS 56 d的大鼠粘膜层显示正常（expt.（3）结论：这些研究结果表明，在大鼠中由FOS诱导的盲肠伊加浓度的瞬时增加与肠道通透性增加引起的粘膜炎症有关;这些可能是由盲肠粘液屏障的破坏引起的。观察到的反应可能有助于肠道免疫系统的成熟。
Background: The mechanism underlying transient increases in immunoglobulin (Ig) A concentrations in the cecal contents of rats fed fructo-oligosaccharide (FOS) is unclear.Objective: This study was designed to test whether increased IgA concentrations represent one aspect of the inflammatory response to increased permeability induced by FOS in the cecum.Methods: Seven-week-old male Wistar rats were fed a fiber-free semipurified diet (FFP) with or without supplemental FOS (60 g/kg diet) for 9 or 58 d [experiment (expt.) 1], 7 d (expt. 2), or 7 or 56 d (expt. 3). In addition to measuring IgA concentrations in cecal content, we assessed gut permeability, inflammatory responses (expt. 1), the number of IgA plasma cells in the cecal lamina propria, polymeric Ig receptor (pIgR) expression in the cecal mucosa (expt. 2), and the condition of the cecal mucus layer (expt. 3).Results: The cecal IgA concentration in the FOS-fed rats was 15-fold higher than that of the rats fed FFP for 9 d (P < 0.05). Gut permeability estimated by urinary chromium-EDTA excretion, bacterial translocation to mesenteric lymph nodes, myeloperoxidase activity, and expression of inflammatory cytokine genes in the cecal mucosa was greater in the FOS-fed rats than in the rats fed FFP for 9 d. These effects were not observed in the rats fed FOS for 58 d (expt. 1). Accompanying the higher cecal IgA concentration, pIgR protein and the number of IgA plasma cells in the cecal mucosa were higher in the FOS-fed rats than in the rats fed FFP for 7 d (expt. 2). Destruction of the mucus layer on the epithelial surface, as evidenced by Alcian blue staining in the cecal sections, was evident in the rats fed FOS for 7 d, but themucus layer appeared normal in the rats fed FOS for 56 d (expt. 3).Conclusions: These findings suggest that transient increases in cecal IgA concentrations induced by FOS in rats are associated with mucosal inflammation in response to increased gut permeability; these are presumably evoked by disruption of the cecal mucus barrier. The observed responses could contribute to the maturation of the gut immune system.