Determination Method for Pyrroloquinoline Quinone in Food Products by HPLC-UV Detection Using a Redox-Based Colorimetric Reaction
Determination Method for Pyrroloquinoline Quinone in Food Products by HPLC-UV Detection Using a Redox-Based Colorimetric Reaction
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DOI：
10.1248/cpb.c21-00638
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发表时间：
2022-01-01
影响因子：
1.7
通讯作者：
Kuroda, Naotaka
中科院分区：
文献类型：
--
作者：
Fukuda, Mizuho;Kishikawa, Naoya;Kuroda, Naotaka
我们开发了一种 HPLC-UV 方法来测定吡咯喹啉醌 (PQQ)，该方法利用基于氧化还原的比色反应。在所提出的比色反应中，PQQ 和二硫苏糖醇之间的氧化还原反应产生超氧阴离子自由基，可以将 2-(4-碘苯基)-3-(4-硝基苯基)-5-苯基-2H-氯化四唑 (INT) 转化为染料。 PQQ 在十八烷基硅胶柱上分离后，与二硫苏糖醇和 INT 在线混合，并通过 490 nm 处的吸光度监测形成的甲臜染料。该方法的检测限（S/N=3）为7.6nM（152 fmol/注射）。所提出的方法可以选择性地检测食品中的 PQQ，无需任何清理程序。
We have developed an HPLC-UV method for the determination of pyrroloquinoline quinone (PQQ), which utilizes a redox-based colorimetric reaction. In the proposed colorimetric reaction, the redox reaction between PQQ and dithiothreitol generates superoxide anion radicals that can convert 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride (INT) to formaz an dye. After PQQ separation on an octadecyl silica column, it was mixed online with dithiothreitol and INT, and the formed formazan dye was monitored by absorbance at 490nm. The detection limit (S/N=3) of the proposed method was 7.6nM (152 fmol/injection). The proposed method could selectively detect PQQ in food products without any clean-up procedures.