CD44v6 expression in inflammatory bowel disease is associated with activity detected by endoscopy and pathological features
CD44v6 expression in inflammatory bowel disease is associated with activity detected by endoscopy and pathological features
复制标题
DOI：
10.1046/j.1365-2559.1999.00712.x
复制
发表时间：
1999-08-01
期刊：
影响因子：
6.4
通讯作者：
Rodríguez, R
中科院分区：
文献类型：
--
作者：
Camacho, FI;Muñoz, C;Rodríguez, R
目的：CD44v6在结肠隐窝上皮细胞中的过表达被认为对溃疡性结肠炎与其他形式的结肠炎(包括克罗恩病)有鉴别诊断价值。目的：探讨CD44v6在炎症性肠病(IBD)中的表达及其与疾病活动性之间的关系。方法与结果：采用免疫组织化学方法检测了100例溃疡性结肠炎(71例)和克罗恩病(29例)手术和内窥镜下结肠组织中CD44v6的表达，并通过胃镜和显微镜检查了解疾病活动性。71例溃疡性结肠炎标本中55例(77.5%)显示单抗2F10染色，29例克罗恩病标本中16例(55.2%)结肠上皮染色。CD44v6阳性率为88.2%(15/17)，CD44v6表达与IBD活动性密切相关(P=0.007)。结论：CD44v6表达与IBD的活动性及病理特征密切相关。我们的数据表明，CD44v6的表达与其他因素一起作为评估疾病活动性的手段可能有一定的用处。此外，CD44v6在溃疡性结肠炎中的表达高于克罗恩病(P=0.02)，尽管这并不能证实单抗2F10在溃疡性结肠炎和克罗恩病的鉴别诊断中的作用，因为克罗恩病中有显著的阳性标本。
Aims: Overexpression of CD44v6 in colon crypt epithelial cells has been suggested to have diagnostic potential in differentiating ulcerative colitis from other forms of colon inflammation, including Crohn's disease. Our aim was to determine the value of CD44v6 expression in inflammatory bowel disease (IBD) and to look for possible associations between CD44v6 expression and activity of this disease.Methods and results: CD44v6 expression was studied using immunohistochemical techniques in 100 surgical and endoscopic colon samples of ulcerative colitis (n=71) and Crohn's disease (n=29), and in every case disease activity was studied by endoscopy and microscopic examination. Fifty-five of 71 (77.5%) samples of ulcerative colitis showed monoclonal antibody 2F10 stained colon epithelium, as did 16 of 29 (55.2%) samples of Crohn's disease. CD44v6 was detected in 88.2% (15 of 17) of cases of IBD with severe disease activity and in 100% of eight cases of severe ulcerative colitis, Our study showed a strong association between CD44v6 expression and the activity of IBD (P = 0.007).Conclusions: CD44v6 expression in IBD is significantly associated with activity detected by means of endoscopy and pathological features. Our data suggest that CD44v6 expression may have some usefulness in conjunction with other factors as a means of evaluating the disease activity. Moreover, CD44v6 expression was higher in ulcerative colitis than Crohn's disease (P=0.02), although this does not confirm the utility of monoclonal antibody 2F10 in differential diagnosis between ulcerative colitis and Crohn's disease, as there was a notable percentage of positive samples of Crohn's disease.