Interferon potentiates cytotoxic effects of 5-fluorouracil on cell proliferation of established human cell lines originating from neoplastic tissues.
Interferon potentiates cytotoxic effects of 5-fluorouracil on cell proliferation of established human cell lines originating from neoplastic tissues.
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干扰素增强 5-氟尿嘧啶对源自肿瘤组织的已建立人类细胞系的细胞增殖的细胞毒性作用。
DOI：
10.1016/0304-3835(83)90160-x
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发表时间：
1983
期刊：
影响因子：
9.7
通讯作者：
Masayoshi Namba
中科院分区：
文献类型：
--
作者：
Toshihiro Miyoshi;Shigeo Ogawa;Toshinori Kanamori;M. Nobuhara;Masayoshi Namba
研究了干扰素增强5-氟尿嘧啶(5-FU)对人肿瘤细胞的细胞毒作用。所用的人肿瘤细胞系为HeLa(子宫颈癌)、MCF-7(乳腺癌)、WI-38CT-1(经Co-60伽玛射线照射转化的胚胎肺成纤维细胞)、KMM-1(骨髓瘤)和Raji(Burkitt淋巴瘤)。所用的正常人细胞株为WI-38(正常人肺成纤维细胞)。通过对HeLa、MCF-7、WI-38CT-1和WI-38细胞的集落形成以及对KMM-1和Raji细胞的生长来确定其细胞毒作用。每种细胞对干扰素或5-FU的敏感性不同。干扰素协同增强5-FU对HeLa、WI-38、CT-1和KMM-1细胞的细胞毒作用。在Raji细胞中，干扰素和5-FU的细胞毒作用是相加的。两药联用对MCF-7和WI-38细胞均无协同或相加致死作用。目前的结果表明，干扰素和5-FU可以突变地减少治疗癌症患者所需的其他药物的量。
A potentiation of the cytotoxic effects of 5-fluorouracil (5-FU) on human tumor cells by interferon was examined. The human neoplastic cell lines used were HeLa (uterine cervical cancer), MCF-7 (mammary cancer), WI-38 CT-1 (embryonic lung fibroblasts transformed in culture by Co-60 gamma-ray irradiation), KMM-1 (myeloma) and Raji (Burkitt's lymphoma). The normal human cell strain used was WI-38 (normal human lung fibroblasts). The cytotoxic effects were determined by colony formation for HeLa, MCF-7, WI-38 CT-1 and WI-38 cells, and by cell growth for KMM-1 and Raji cells. Each cell line was different in sensitivity to interferon or 5-FU. Interferon potentiated synergistically the cytotoxic effects of 5-FU on HeLa, WI-38 CT-1 and KMM-1 cells. In the case of Raji cells, the cytotoxic effects of the combination of interferon and 5-FU were additive. Neither synergistic nor additive lethal effects of the combination of the 2 agents were observed in MCF-7 and WI-38 cells. The present results indicate a possibility that interferon and 5-FU can mutally reduce the amount of the other needed to treat cancer patients.
胸苷、5-氟尿嘧啶和脱氧胞苷对培养的 HeLa 细胞的细胞毒性和细胞因子作用。
影响因子：
11.2
作者：
Fried,J;Perez,AG;Doblin,JM;Clarkson,BD
通讯作者：
Clarkson,BD
通过正常人血清进行组织培养脱髓鞘。
影响因子：
11.2
作者：
Silberberg,DH;Manning,MC;Schreiber,AD
通讯作者：
Schreiber,AD