ASSESSMENT OF PARASITE POPULATION-DYNAMICS IN MIXED INFECTIONS OF RODENT PLASMODIA
ASSESSMENT OF PARASITE POPULATION-DYNAMICS IN MIXED INFECTIONS OF RODENT PLASMODIA
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DOI：
10.1017/s0031182000074539
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发表时间：
1992-12-01
期刊：
影响因子：
2.4
通讯作者：
BROWN, KN
中科院分区：
文献类型：
--
作者：
SNOUNOU, G;BOURNE, T;BROWN, KN
用来自查鲍迪疟原虫基因组文库的PCsv4.1探针，经Southern blotting产生的RFLP图谱可以区分这四种啮齿动物疟原虫的克隆株。用来自两个寄生虫种或株的克隆株同时接种CBA/Ca小鼠。利用啮齿动物疟疾品系启动了6个混合物种和3个混合菌株的感染。通过分析从日常血液样本中提纯的DNA，定性和半定量地确定了每组寄生虫种群的组成，从而提供了每种混合感染的动态表示。对寄生虫病病程的影响进行了介绍和讨论。
Cloned lines of the four rodent Plasmodium species can be differentiated by the RFLP pattern generated following Southern blotting and probing with PCsv4.1, a probe derived from a P. chabaudi chabaudi genomic library. Groups of CBA/Ca mice were inoculated simultaneously with cloned lines from two parasite species or strains. Six mixed species and three mixed strain infections using rodent malaria lines were initiated. The composition of the parasite population in each group was determined qualitatively and semi-quantitatively by analysis of the DNA purified from daily blood samples, thereby providing a dynamic representation of each mixed infection. Effects on the course of parasitaemias are presented and discussed.