Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli
Effect of tandemly repeated AGG triplets on the translation of CAT‐mRNA in E. coli
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DOI：
10.1016/0014-5793(92)80761-5
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发表时间：
1992-07
期刊：
影响因子：
3.5
通讯作者：
I. Ivanov;I. Ivanov;R. Alexandrova;B. Dragulev;A. Saraffova;M. AbouHaidar
中科院分区：
文献类型：
--
作者：
I. Ivanov;I. Ivanov;R. Alexandrova;B. Dragulev;A. Saraffova;M. AbouHaidar
研究表明，稀有精氨酸密码子AGG的串联对mRNA在大肠杆菌中的翻译有很强的抑制作用。coli[5]。这可以通过这些密码子与丰度较低的tRNAAGG的限速相互作用来解释[6]。在这项研究中，串联重复的AGG三联体被引入到氯霉素乙酰转移酶（CAT）基因的起始ATG密码子的上游或下游（包括框内和框外），并研究修饰基因的表达。我们报告说，除了AGG集群导致CAT基因表达的实质性抑制作用，独立于它们在mRNA中的定位。这种抑制作用可以通过串联AGGAGG与天然Shine-Dalgarno（SD）序列（共有序列AAGGAGGU）竞争16 S小核糖体RNA（rRNA）的3′端来解释。
It has been shown that tandems of rare arginine codons AGG have a strong inhibitory effect on translation of mRNA inE. coli[5]. This has been explained by the rate-limiting interaction of these codons with the less abundant tRNAAGG[6]. In this study tandemly repeated AGG triplets were introduced into the chloramphenicol acetyltransferase (CAT) gene either upstream of the initiation ATG codon or downstream of it (both in frame and out of frame) and the expression of the modified genes was investigated. We report that the addition of AGG clusters resulted in a substantial inhibitory effect on CAT gene expression independently of their localization in mRNA. This inhibitory effect is explained by a competition of the tandem AGGAGG with the natural Shine—Dalgarno (SD) sequence (consensus AAGGAGGU) for the 3′-end of the 16S small ribosomal RNA (rRNA).