Estrogen influences satellite cell activation and proliferation following downhill running in rats
Estrogen influences satellite cell activation and proliferation following downhill running in rats
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DOI：
10.1152/japplphysiol.00128.2007
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发表时间：
2008-02-01
影响因子：
3.3
通讯作者：
Tiidus, Peter M.
中科院分区：
文献类型：
--
作者：
Enns, Deborah L.;Tiidus, Peter M.
为了研究雌激素对运动后肌肉修复过程的影响，我们检查了补充雌激素（0.25 毫克颗粒）对下坡跑 72 小时后大鼠骨骼肌中总卫星细胞、活化卫星细胞和增殖卫星细胞标记呈阳性的肌纤维数量的影响。切除卵巢的雌性大鼠（n = 44）分为四组（每组 n = 11）：假手术（无雌激素）对照组（SC）；假的，锻炼的（SE）；补充雌激素的对照（EC）；补充雌激素并锻炼（EE）。雌激素暴露 8 天后，动物在跑步机上以 17 m/min (- 13.5) 的速度运行 90 分钟。 72 小时后，取出比目鱼肌和股白肌，并对总的[配对盒同源异型基因 7 (Pax7)]、[活化的肌源分化因子 D (MyoD)] 和增殖的 [5-溴-2'-脱氧尿苷 (BrdU)] 卫星细胞进行免疫染色。运动后两块肌肉的 β-葡萄糖醛酸酶活性均增加 (P < 0.05)；然而，与 SE 组相比，EE 组运动后比目鱼肌酶活性的升高有所减弱（P < 0.05）。免疫组织化学分析显示，与对照组相比，运动组显示出含有总卫星细胞、活化卫星细胞和增殖卫星细胞的肌纤维数量增加（P < 0.05）。此外，与 SE 动物相比，运动后 EE 动物的两块肌肉中观察到更多数量的总卫星细胞、活化卫星细胞和增殖卫星细胞标记呈阳性的纤维（P < 0.05）。结果表明，雌激素可能通过激活卫星细胞来影响骨骼肌损伤后的修复。
To investigate the influence of estrogen on postexercise muscle repair processes, we examined the effects of estrogen supplementation (0.25-mg pellet) on numbers of myofibers positive for markers of total, activated, and proliferating satellite cells in rat skeletal muscles 72 h following downhill running. Ovariectomized female rats (n = 44) were divided into four groups (n = 11 per group): sham (no estrogen) controls (SC); sham, exercised (SE); estrogen-supplemented controls (EC); and estrogen-supplemented, exercised (EE). After 8 days of estrogen exposure, animals were exposed to 90 min of treadmill running at 17 m/min (- 13.5). Seventy-two hours later, soleus and white vastus muscles were removed and immunostained for total [paired box homeotic gene 7 (Pax7)], [activated myogenic differentiation factor D (MyoD)], and proliferating [5-bromo-2'-deoxyuridine (BrdU)] satellite cells. beta-Glucuronidase activity was increased (P < 0.05) in both muscles following exercise; however, the postexercise elevations in enzyme activity were attenuated in the EE group compared with the SE group in the soleus (P < 0.05). Immunohistochemical analysis revealed that exercised groups displayed increased numbers of myofibers containing total, activated, and proliferating satellite cells compared with control groups (P < 0.05). Furthermore, greater numbers of fibers positive for markers of total, activated, and proliferating satellite cells were observed postexercise in EE animals compared with SE animals for both muscles (P < 0.05). The results demonstrate that estrogen may potentially influence postdamage repair of skeletal muscle through activation of satellite cells.