EFFECTS OF VITAMIN-A-DEFICIENCY AND REPLETION ON RAT INSULIN-SECRETION INVIVO AND INVITRO FROM ISOLATED ISLETS
EFFECTS OF VITAMIN-A-DEFICIENCY AND REPLETION ON RAT INSULIN-SECRETION INVIVO AND INVITRO FROM ISOLATED ISLETS
复制标题
DOI：
10.1172/jci112778
复制
发表时间：
1987-01-01
影响因子：
15.9
通讯作者：
MEDA, P
中科院分区：
文献类型：
--
作者：
CHERTOW, BS;BLANER, WS;MEDA, P
本文研究了维生素A缺乏和补充对大鼠胰岛素释放和胰岛细胞视黄醇结合蛋白（CRBP）和细胞视黄酸结合蛋白（CRABP）的影响。维生素A缺乏的灌注胰岛的双相胰岛素释放明显受损。与从以棕榈酸视黄酯形式的视黄醇充满的大鼠胰岛的释放（4 μ g/g饮食）相比，释放在视黄酸（RA）充满时（2 μ g/g饮食）仍然受损。用RA，8 μ g/g饮食标准化释放。维生素A缺乏不影响胰岛胰岛素含量、细胞大小、数量或结构。在体内，维生素A缺乏的大鼠有受损的葡萄糖诱导的急性胰岛素释放和葡萄糖耐受不良，这改善与饱食。正常胰岛的CRBP浓度高于CRABP;维生素A缺乏降低CRBP，但不降低CRABP水平。我们的结论是，视黄醇是正常的胰岛素分泌所必需的。视黄酸可代替视黄醇发挥这一功能。
We studied the effects of vitamin A deficiency and repletion on rat insulin release and islet cellular retinol binding protein (CRBP) and cellular retinoic acid binding protein (CRABP). Biphasic insulin release from vitamin A-deficient perifused islets was markedly impaired. Release remained impaired with retinoic acid (RA) repletion, 2 .mu.g/g diet compared to release from islets of rats repleted with retinol in the form of retinyl palmitate, 4 .mu.g/g diet. Release normalized with RA, 8 .mu.g/g diet. Vitamin A deficiency did not affect islet insulin content, cell size, number or structure. In vivo, vitamin A-deficient rats had impaired glucose-induced acute insulin release and glucose intolerance, which improved with repletion. Normal islets had greater concentrations of CRBP than CRABP; vitamin A deficiency reduced CRBP but not CRABP levels. We conclude retinol is required for normal insulin secretion. Retinoic acid may substitute for retinol in this function.