A novel in vivo murine model of cartilage regeneration. Age and strain-dependent outcome after joint surface injury.
A novel in vivo murine model of cartilage regeneration. Age and strain-dependent outcome after joint surface injury.
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DOI：
10.1016/j.joca.2008.11.003
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发表时间：
2009-06
影响因子：
7
通讯作者：
Dell'accio F
中科院分区：
文献类型：
--
作者：
Eltawil NM;De Bari C;Achan P;Pitzalis C;Dell'accio F
建立并验证急性机械损伤后关节表面修复的小鼠模型。通过显微外科手术在C57 BL/6和DBA/1小鼠的髌骨沟中产生全层缺损。对照组膝关节为假手术组或非手术组。结果通过组织学评分系统进行评价。TUNEL法检测细胞凋亡，Phospho-Histone H3染色检测细胞增殖。II型胶原蛋白新沉积和降解分别通过使用针对CPII端肽和C1，2C（Col 2 -3/4Cshort）的抗体的免疫染色进行评价。通过对TEGE 373和VDIPEN新表位进行免疫染色来评估聚集蛋白聚糖酶和基质金属蛋白酶（MMPs）活性。年轻的8周龄DBA/1小鼠表现出一致的和优越的关节软骨缺损愈合的上级。C57 BL/6小鼠的骨关节炎（OA）修复不良，并发展为骨关节炎（OA）的特征。与C57 BL/6相比，DBA/1小鼠的软骨细胞凋亡、修复组织内的细胞增殖、持续的II型胶原蛋白新沉积、II型胶原蛋白降解减少、聚集蛋白聚糖酶减少和MMP诱导的聚集蛋白聚糖降解增加。8个月大的DBA/1小鼠未能修复，但与年龄匹配的C57 BL/6小鼠相反，没有出现OA的迹象。我们已经建立并验证了一种小鼠软骨再生模型，其中关节表面损伤的结果是应变和年龄依赖性的。该模型将首次允许使用强大的小鼠遗传学技术解剖成年哺乳动物关节表面再生所涉及的不同途径。
To generate and validate a murine model of joint surface repair following acute mechanical injury. Full thickness defects were generated in the patellar groove of C57BL/6 and DBA/1 mice by microsurgery. Control knees were either sham-operated or non-operated. Outcome was evaluated by histological scoring systems. Apoptosis and proliferation were studied using TUNEL and Phospho-Histone H3 staining, respectively. Type II collagen neo-deposition and degradation were evaluated by immunostaining using antibodies to the CPII telopeptide and C1,2C (Col2-3/4Cshort), respectively. Aggrecanases and matrix metalloproteinases (MMPs) activity were assessed by immunostaining for TEGE373 and VDIPEN neo-epitopes. Young 8-week-old DBA/1 mice displayed consistent and superior healing of the articular cartilage defect. Age-matched C57BL/6 mice repaired poorly and developed features of osteoarthritis (OA). Compared to C57BL/6, DBA/1 mice displayed a progressive decline of chondrocyte apoptosis, cell proliferation within the repair tissue, persistent type II collagen neo-deposition, less type II collagen degradation, less aggrecanases and more MMP-induced aggrecan degradation. Eight-month-old DBA/1 mice failed to repair, but, in contrast to age-matched C57BL/6 mice, developed no signs of OA. We have generated and validated a murine model of cartilage regeneration in which the outcome of joint surface injury is strain and age dependent. This model will allow, for the first time, the dissection of different pathways involved in joint surface regeneration in adult mammals using the powerful technology of mouse genetics.
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影响因子：
3.2
作者：
Lee, ER;Smith, CE;Poole, AR
通讯作者：
Poole, AR
DOI：
10.1053/jars.2002.32839
发表时间：
2002-09-01
影响因子：
4.7
作者：
Hjelle, K;Solheim, E;Brittberg, M
通讯作者：
Brittberg, M
DOI：
10.1016/s0749-8063(97)90124-9
发表时间：
1997-08-01
影响因子：
4.7
作者：
Curl, WW;Krome, J;Poehling, GG
通讯作者：
Poehling, GG
影响因子：
15.9
作者：
Little, Christopher B.;Meeker, Clare T.;Fosang, Amanda J.
通讯作者：
Fosang, Amanda J.
影响因子：
--
作者：
Ding, CH;Cicuttini, FM;Jones, G
通讯作者：
Jones, G