Helicobacter pylori patient isolates from South Africa and Nigeria differ in virulence factor pathogenicity profile and associated gastric disease outcome
Helicobacter pylori patient isolates from South Africa and Nigeria differ in virulence factor pathogenicity profile and associated gastric disease outcome
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DOI：
10.1038/s41598-020-66128-0
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发表时间：
2020-07-10
影响因子：
4.6
通讯作者：
Haas, Rainer
中科院分区：
文献类型：
--
作者：
Palamides, Pia;Jolaiya, Tolulope;Haas, Rainer
幽门螺杆菌（Helicobacter pylori，Hp）是一种革兰氏阴性螺旋状病原菌，是胃炎、消化性溃疡的主要致病菌，被世界卫生组织列为I类致癌物。而H.非洲是世界上幽门螺杆菌感染率最高的国家之一，胃癌的发病率也很低。关于H相关的其他症状知之甚少。非洲的幽门螺杆菌感染以及与某些细菌毒力表型的相关性。我们在尼日利亚（NG）和南非（ZA）建立了一个研究中心网络，以获得流行病学情况的概述。共分析了来自114例患者的220株分离株，并检查了118株不同患者分离株是否存在毒力因子cagA、vacA、dupA、其系统发育起源及其对常用抗生素阿莫西林、克拉霉素、甲硝唑和四环素的耐药性。我们报告说，H。来自尼日利亚和南非的幽门螺杆菌分离株在它们的系统发育谱和它们的毒力因子表达方面存在显著差异。hpAfrica 2菌株中VacA嵌合现象强烈，导致m1-m2 vacA嵌合体和频繁的s1 m1和s1 m2 vacA亚型。胃病变在尼日利亚患者中比在南非患者中更常见，H。对一种或多种抗生素具有耐药性的幽门螺杆菌分离株在这两个国家中经常出现。
Helicobacter pylori is a gram-negative, spiral-shaped bacterial pathogen and the causative agent for gastritis, peptic ulcer disease and classified as a WHO class I carcinogen. While the prevalence of H. pylori infections in Africa is among the highest in the world, the incidence of gastric cancer is comparably low. Little is known about other symptoms related to the H. pylori infection in Africa and the association with certain phenotypes of bacterial virulence. We established a network of study sites in Nigeria (NG) and South Africa (ZA) to gain an overview on the epidemiological situation. In total 220 isolates from 114 patients were analyzed and 118 different patient isolates examined for the presence of the virulence factors cagA, vacA, dupA, their phylogenetic origin and their resistance against the commonly used antibiotics amoxicillin, clarithromycin, metronidazole and tetracycline. We report that H. pylori isolates from Nigeria and South Africa differ significantly in their phylogenetic profiles and in their expression of virulence factors. VacA mosaicism is intensive, resulting in m1-m2 vacA chimeras and frequent s1m1 and s1m2 vacA subtypes in hpAfrica2 strains. Gastric lesions were diagnosed more frequent in Nigerian versus South African patients and H. pylori isolates that are resistant against one or multiple antibiotics occur frequently in both countries.