Isolation of Elizabethkingia anophelis From COVID-19 Swab Kits.
Isolation of Elizabethkingia anophelis From COVID-19 Swab Kits.
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从 COVID-19 拭子套件中分离伊丽莎白金氏按蚊。
DOI：
10.3389/fmicb.2021.799150
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发表时间：
2021
影响因子：
5.2
通讯作者：
Shi X
中科院分区：
文献类型：
--
作者：
Xu L;Peng B;He Y;Cui Y;Hu Q;Wu Y;Chen H;Zhou X;Chen L;Jiang M;Zuo L;Chen Q;Wu S;Liu Y;Qin Y;Shi X
目的：调查和表征COVID-19大流行期间，从中国深圳三个与COVID-19无关的发热疫情聚集区患者的咽拭子和肛门拭子样本中分离出的疑似按蚊嗜热金虫污染物。方法：对28例发热青少年患者的咽拭子（n = 28）和肛拭子（n = 3）进行细菌培养。采用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF/MS）和VITEK 2自动鉴定系统对分离的细菌菌株进行鉴定。从患者样本（n = 31）、来自与患者样本相同制造商的未开封病毒采集试剂盒（n = 35，空白样本）和来自其他两个制造商的未开封咽拭子采集试剂盒（n = 22，对照样本）中提取核酸。进行宏基因组测序和定量实时PCR（qPCR）检测。从患者（n = 13）收集的血清中评估是否存在抗E.按蚊对大肠杆菌的基因组特征、抗生素敏感性和耐热性进行了研究。按蚊分离株（n = 31）进行了分析。结果：分离株经MALDI-TOF/MS和VITEK 2鉴定为脑膜炎败血嗜沙金杆菌。DNA序列分析证实分离株为E.按蚊患者标本和空白标本均为E.按蚊对照样品为E阴性。按蚊13名患者的血清对分离的E.按蚊大多数分离株高度同源，携带多种β-内酰胺酶基因（blaB、blaGOB和blaCME）。分离株对硝基呋喃类、青霉素类和大多数β-内酰胺类药物耐药。细菌在56°C下加热30分钟后存活。结论：同一厂家生产的未开封的市售病毒采集试剂盒存在感染大肠杆菌的情况。按蚊患者均未感染E.按蚊和发烧的病原体仍未查明。这一发现迅速通知了有关当局，随后的采集包没有受到污染。基于DNA序列的鉴定技术是目前鉴定黄喉盘菌属物种的权威方法。急诊按蚊分离株具有多重抗药性，并具有部分耐热性，这使得它们难以从受污染的表面上根除。这种耐药性表明，应更加重视消毒方案，特别是在医院，以避免暴发流行的E。按蚊感染
Purpose: To investigate and characterize the putative Elizabethkingia anophelis contaminant isolated from throat and anal swab samples of patients from three fever epidemic clusters, which were not COVID-19 related, in Shenzhen, China, during COVID-19 pandemic. Methods: Bacteria were cultured from throat (n = 28) and anal (n = 3) swab samples from 28 fever adolescent patients. The isolated bacterial strains were identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS) and the VITEK2 automated identification system. Nucleic acids were extracted from the patient samples (n = 31), unopened virus collection kits from the same manufacturer as the patient samples (n = 35, blank samples) and from unopened throat swab collection kits of two other manufacturers (n = 22, control samples). Metagenomic sequencing and quantitative real-time PCR (qPCR) detection were performed. Blood serum collected from patients (n = 13) was assessed for the presence of antibodies to E. anophelis. The genomic characteristics, antibiotic susceptibility, and heat resistance of E. anophelis isolates (n = 31) were analyzed. Results: The isolates were identified by MALDI-TOF/MS and VITEK2 as Elizabethkingia meningoseptica. DNA sequence analysis confirmed isolates to be E. anophelis. The patients’ samples and blank samples were positive for E. anophelis. Control samples were negative for E. anophelis. The sera from a sub-sample of 13 patients were antibody-negative for isolated E. anophelis. Most of the isolates were highly homologous and carried multiple β-lactamase genes (blaB, blaGOB, and blaCME). The isolates displayed resistance to nitrofurans, penicillins, and most β-lactam drugs. The bacteria survived heating at 56°C for 30 min. Conclusion: The unopened commercial virus collection kits from the same manufacturer as those used to swab patients were contaminated with E. anophelis. Patients were not infected with E. anophelis and the causative agent for the fevers remains unidentified. The relevant authorities were swiftly notified of this discovery and subsequent collection kits were not contaminated. DNA sequence-based techniques are the definitive method for Elizabethkingia species identification. The E. anophelis isolates were multidrug-resistant, with partial heat resistance, making them difficult to eradicate from contaminated surfaces. Such resistance indicates that more attention should be paid to disinfection protocols, especially in hospitals, to avoid outbreaks of E. anophelis infection.
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DOI：
10.1016/j.ijmm.2013.02.007
发表时间：
2013-08
期刊：
International journal of medical microbiology : IJMM
影响因子：
--
作者：
Otto M
通讯作者：
Otto M
影响因子：
11.8
作者：
Yung CF;Maiwald M;Loo LH;Soong HY;Tan CB;Lim PK;Li L;Tan NW;Chong CY;Tee N;Thoon KC;Chan YH
通讯作者：
Chan YH
影响因子：
1.3
作者：
Govindaswamy, Aishwarya;Bajpai, Vijeta;Mathur, Purva
通讯作者：
Mathur, Purva
影响因子：
2.3
作者：
Kyritsi, Maria A.;Mouchtouri, Varvara A.;Hadjichristodoulou, Christos
通讯作者：
Hadjichristodoulou, Christos
影响因子：
11.8
作者：
Moore LS;Owens DS;Jepson A;Turton JF;Ashworth S;Donaldson H;Holmes AH
通讯作者：
Holmes AH