ISOLATION OF RUBELLA-VIRUS FROM BRAIN IN CHRONIC PROGRESSIVE PANENCEPHALITIS
ISOLATION OF RUBELLA-VIRUS FROM BRAIN IN CHRONIC PROGRESSIVE PANENCEPHALITIS
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DOI：
10.1099/0022-1317-29-2-143
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发表时间：
1975-01-01
影响因子：
3.8
通讯作者：
CARNAY, L
中科院分区：
文献类型：
--
作者：
CREMER, NE;OSHIRO, LS;CARNAY, L
从一名先天性感染的 12 岁儿童的大脑中分离出风疹病毒，该儿童分别在 8 岁时和 11 岁时出现明显的进行性智力和运动退化。该病毒最初是在 CV-1 细胞与胰蛋白酶处理的脑组织共培养物中分离出来的；随后，脑组织培养物也显示出风疹病毒感染的证据，通过间接荧光抗体技术（IFA）使用在兔子中制备的抗风疹病毒抗体作为中间血清来识别。两种分离株均干扰埃可病毒11型对BSC-1细胞系的感染。IFA用特异性抗血清将干扰病毒鉴定为风疹病毒，并将其命名为风疹病毒NTr株。血清中针对风疹病毒的补体固定抗体滴度为1:256。脊髓液是抗补体的。血清中风疹病毒血凝抗体滴度（HI）为1:8196，脊髓液中为1:128。 HI 抗体属于 IgG 类。血清和脊髓液中与风疹病毒相应的HI滴度分别为1:8和<1:2。
Rubella virus was isolated from the brain of a congenitally-infected, 12-year-old child in whom progressive mental and motor deterioration became evident at age 8 and 11 years respectively. The virus was initially isolated in a co-culture of CV-1 cells with the trypsinized brain tissue; subsequently the culture of the brain tissue also showed evidence of rubella virus infection recognized by indirect fluorescent antibody technique (IFA) using anti-rubella virus antibody prepared in rabbits as intermediate serum. Both isolates interfered with infection of BSC-1 cell lines by echovirus type 11. The interfering virus was identified as rubella virus by IFA with the specific antiserum, and it is designated as the NTr strain of rubella virus. The complement fixing antibody titre to rubella virus in serum was 1:256. The spinal fluid was anticomplementary. Rubella virus haemagglutinating antibody titre (HI) in serum was 1:8196 and in the spinal fluid 1:128. The HI antibody was of the IgG class. The corresponding HI titres to rubeola virus in serum and spinal fluid were 1:8 and < 1:2 respectively.