Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses
Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses
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DOI：
10.1126/science.282.5393.1490
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发表时间：
1998-11-20
期刊：
影响因子：
56.9
通讯作者：
Sancar, A
中科院分区：
文献类型：
--
作者：
Thresher, RJ;Vitaterna, MH;Sancar, A
隐花色素是眼睛中的光敏色素，已被提议用作昼夜节律感光色素。测试了缺乏隐花色素 2 蓝光感光基因 (mCry2) 的小鼠的生物钟相关功能。突变小鼠对视交叉上核 (SCN) 中 mPer1 的急性光诱导敏感性较低，但 SCN 中 mPer1 和 mCry1 信使 RNA 表现出正常的昼夜节律振荡。在行为上，突变体的内在昼夜节律周期比正常长约 1 小时，并且响应于昼夜节律时间 17 施用的光脉冲而表现出高振幅相移。这些数据与 CRY2 蛋白调节小鼠昼夜节律反应的假设一致，并表明隐花色素在哺乳动物的昼夜节律光感受中发挥作用。
Cryptochromes are photoactive pigments in the eye that have been proposed to function as circadian photopigments. Mice lacking the cryptochrome 2 blue-light photoreceptor gene (mCry2) were tested for circadian clock-related functions. The mutant mice had a lower sensitivity to acute Light induction of mPer1 in the suprachiasmatic nucleus (SCN) but exhibited normal circadian oscillations of mPer1 and mCry1 messenger RNA in the SCN. Behaviorally, the mutants had an intrinsic circadian period about 1 hour Longer than normal and exhibited high-amplitude phase shifts in response to Light pulses administered at circadian time 17. These data are consistent with the hypothesis that CRY2 protein modulates circadian responses in mice and suggest that cryptochromes have a role in circadian photoreception in mammals.