Chemical and biological insecticides select distinct gene expression patterns in Aedes aegypti mosquito
Chemical and biological insecticides select distinct gene expression patterns in Aedes aegypti mosquito
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DOI：
10.1098/rsbl.2014.0716
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发表时间：
2014-12-01
期刊：
影响因子：
3.3
通讯作者：
David, Jean-Philippe
中科院分区：
文献类型：
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作者：
Despres, Laurence;Stalinski, Renaud;David, Jean-Philippe
蚊子对化学杀虫剂的抗药性在世界范围内的演变是对公共卫生的一个重大挑战，病媒控制的未来在很大程度上取决于可与化学品结合使用的生物杀虫剂的开发（综合管理），预计已经对化学品产生抗药性的种群不会轻易对生物杀虫剂产生抗药性。然而，很少有人知道受化学或生物杀虫剂选择影响的代谢途径。在这里，我们表明，埃及伊蚊，一个实验室蚊子菌株选择与生物杀虫剂（苏云金芽孢杆菌以色列，BTI）进化增加转录的许多基因编码的内肽酶，而大多数基因编码的解毒酶表达不足。相比之下，在用化学物质选择的菌株中，编码解毒酶的基因大多过表达。在所有抗性菌株中，参与免疫应答的基因转录不足，这表明基础免疫可能是补偿杀虫剂选择引起的代谢成本的一般调节变量。生物测定通常没有显示出选定菌株对其他杀虫剂类型的敏感性增加的证据，所有耐化学品菌株对Bti的敏感性与亲菌株一样，这是对耐化学品的蚊子种群进行可持续综合管理的良好前提。
Worldwide evolution of mosquito resistance to chemical insecticides represents a major challenge for public health, and the future of vector control largely relies on the development of biological insecticides that can be used in combination with chemicals (integrated management), with the expectation that populations already resistant to chemicals will not become readily resistant to biological insecticides. However, little is known about the metabolic pathways affected by selection with chemical or biological insecticides. Here we show that Aedes aegypti, a laboratory mosquito strain selected with a biological insecticide (Bacillus thuringiensis israelensis, Bti) evolved increased transcription of many genes coding for endopeptidases while most genes coding for detoxification enzymes were under-expressed. By contrast, in strains selected with chemicals, genes encoding detoxification enzymes were mostly over-expressed. In all the resistant strains, genes involved in immune response were under-transcribed, suggesting that basal immunity might be a general adjustment variable to compensate metabolic costs caused by insecticide selection. Bioassays generally showed no evidence for an increased susceptibility of selected strains towards the other insecticide type, and all chemical-resistant strains were as susceptible to Bti as the unselected parent strain, which is a good premise for sustainable integrated management of mosquito populations resistant to chemicals.