Interaction of poly(ADP-ribose)polymerase with DNA polymerase α
Interaction of poly(ADP-ribose)polymerase with DNA polymerase α
复制标题
聚（ADP-核糖）聚合酶与 DNA 聚合酶 α 的相互作用
DOI：
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发表时间：
1994
影响因子：
4.3
通讯作者：
C. Simbulan
中科院分区：
文献类型：
--
作者：
S. Yoshida;C. Simbulan
均相纯化的聚（ADP-核糖）聚合酶（PARP）特异性刺激免疫亲和纯化的小牛或人DNA聚合酶α的活性约6至60倍。显然，DNA聚合酶α的聚（ADP-核糖基）化对于刺激不是必需的。PARP对DNA聚合酶α的作用是双相的：在很低的DNA浓度下，PARP抑制其活性，而在较高的DNA浓度下，PARP对DNA聚合酶α有很大的促进作用，PARP的自聚（ADP-核糖基）抑制其促进和抑制作用。通过抗DNA聚合酶α抗体的免疫沉淀，清楚地表明PARP可能与DNA聚合酶α物理相关。DNA聚合酶α的刺激可能归因于两者之间的物理缔合，而不是PARP的DNA结合能力，因为仅含有DNA结合结构域的PARP片段几乎没有刺激活性。小牛胸腺粗提物中也检测到PARP-DNA聚合酶α复合物的存在。
Homogeneously purified poly(ADP-ribose) polymerase (PARP) specifically stimulated the activity of immunoaffinity-purified calf or human DNA polymerase α by about 6 to 60-fold. Apparently, poly(ADP-ribosyl)ation of DNA polymerase α was not necessary for the stimulation. The effects of PARP on DNA polymerase α were biphasic: at very low concentrations of DNA, it rather inhibited its activity, whereas, at higher DNA concentrations, PARP greatly stimulated it. The autopoly(ADP-ribosyl)ation of PARP suppressed both its stimulatory and inhibitory effects. By immunoprecipitation with an anti-DNA polymerase α antibody, it was clearly shown that PARP may be physically associated with DNA polymerase α. Stimulation of DNA polymerase α may be attributed to the physical association between the two, rather than to the DNA-binding capacity of PARP, since the PARP fragment containing only the DNA binding domain showed little stimulatory activity. The existence of PARP-DNA polymerase α complexes were also detected in crude extracts of calf thymus.
聚（ADP-核糖）聚合酶对 SV40 DNA 酶促合成的影响。
DOI：
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发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Eki,T;Hurwitz,J
通讯作者：
Hurwitz,J
通过免疫分级对 SV40 染色质和大 T 抗原的聚（ADP-核糖基化）进行体内表征。
影响因子：
3.7
作者：
Baksi,K;Alkhatib,H;Smulson,ME
通讯作者：
Smulson,ME
具有自身抗体的人聚（ADP-核糖）聚合酶的表征。
DOI：
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发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yamanaka,H;Penning,CA;Willis,EH;Wasson,DB;Carson,DA
通讯作者：
Carson,DA