Aging, sex differences, and oxidative stress in human respiratory and limb muscles
Aging, sex differences, and oxidative stress in human respiratory and limb muscles
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DOI：
10.1016/j.freeradbiomed.2006.05.027
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发表时间：
2006-09-01
影响因子：
7.4
通讯作者：
Gea, Joaquim
中科院分区：
文献类型：
--
作者：
Barreiro, Esther;Coronell, Carlos;Gea, Joaquim
氧化应激与衰老肌肉的少肌症有关。根据骨骼肌是永久活跃的，它们的活动甚至可能随着年龄的增长而增加，我们假设老年健康个体的外部肋间的氧化应激水平可能会增加。我们进行了一项病例对照研究，其中活性羰基，丙二醛-蛋白加合物，3-硝基酪氨酸免疫反应，锰-超氧化物歧化酶（锰-SOD），和过氧化氢酶进行了检测，使用免疫印迹法在外部肋间和四头肌（开放肌肉活检）从12个健康的老年人和12个年轻人的性别。在老年受试者中，反应性羰基，丙二醛-蛋白质加合物，3-硝基酪氨酸，锰-超氧化物歧化酶和过氧化氢酶显着大于在外部intercostals比年轻的控制。对两组男性和女性分别进行的事后分析显示，与对照组女性相比，老年女性的外肋间显示出反应性羰基、丙二醛-蛋白质加合物、3-硝基酪氨酸和Mn-SOD水平显着增加，而老年男性的外肋间则没有。这项研究表明，肌肉活动的差异可能解释了随着年龄的增长，在人类呼吸和肢体肌肉中观察到的氧化应激的差异模式，以及这些肌肉中可能存在与性别相关的这种现象的调节。(c)2006年爱思唯尔公司All rights reserved.
Oxidative stress is involved in the sarcopenia of aging muscles. On the grounds that ventilatory muscles are permanently active, and their activity may even increase with aging, we hypothesized that the levels of oxidative stress would probably be increased in the external intercostals of elderly healthy individuals. We conducted a case-control study in which reactive carbonyl groups, malondialdehyde-protein adducts, 3-nitrotyrosine immunoreactivity, Mn-superoxide dismutase (Mn-SOD), and catalase were detected using immunoblotting in external intercostals and quadriceps (open muscle biopsies) obtained from 12 healthy elderly and 12 young individuals of both sexes. In elderly subjects, reactive carbonyls, malondialdehyde-protein adducts, 3-nitrotyrosine, Mn-SOD, and catalase were significantly greater in the external intercostals than in the young controls. A post hoc analysis, in which men and women from both groups were analyzed separately, revealed that the external intercostals of elderly women, but not those of elderly men, showed significantly increased levels of reactive carbonyls, malondialdehyde-protein adducts, 3-nitrotyrosine, and Mn-SOD compared to those of control females. This study suggests that differences in muscle activity might explain the differential pattern of oxidative stress observed in human respiratory and limb muscles with aging as well as the likely existence of a sex-related regulation of this phenomenon in these muscles. (c) 2006 Elsevier Inc. All rights reserved.