miR-10b-3p, miR-8112 and let-7j as potential biomarkers for autoimmune inner ear diseases
miR-10b-3p, miR-8112 and let-7j as potential biomarkers for autoimmune inner ear diseases
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miR-10b-3p、miR-8112 和 let-7j 作为自身免疫性内耳疾病的潜在生物标志物
DOI：
10.3892/mmr.2019.10248
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发表时间：
2019-05
影响因子：
3.4
通讯作者：
Xu Anting
中科院分区：
文献类型：
--
作者：
Zhang Juhong;Wang Na;Xu Anting
循环microRNA（miRNAs）已被建议作为诊断几种自身免疫性疾病的非侵入性生物标志物。然而，据我们所知，还没有研究检查自身免疫性内耳疾病（AIED）的miRNA表达谱。本研究旨在利用miRNA测序技术检测3只对照小鼠和3只抗原诱导的AIED模型小鼠血清中miRNA的表达谱。使用t检验筛选差异表达的miRNA（DE-miRNA）。使用TargetScan Mouse进行miRNA靶标预测。然后，利用Cytoscape软件构建并可视化了miRNA-靶基因相互作用网络。使用clusterProfiler软件包预测DE-miRNAs靶基因的潜在功能。结果，在AIED和对照小鼠之间鉴定出22个miRNA为DE-miRNAs，包括10个上调和12个下调的基因。基于TargetScan小鼠预测，1，958个基因被鉴定为22个DE-miRNA的靶标。功能分析表明，只有8个miRNAs的靶基因分别富集到Gene Ontology和京都基因与基因组百科全书途径，其中miR-10 b-3 p、let-7 j和miR-8112是两种途径分析共有的。这3种miRNAs可能通过影响炎性趋化因子（miR-10 b-3 p-C-C motif chemokine 12）、Wnt信号传导（miR-8112-Wnt 9 b/Wnt 3a/Wnt 2b）和粘蛋白型O-聚糖生物合成途径（let-7 j-Galnt 2/Galnt 12）参与AIED的发生。结论：miR-10 b-3 p、miR-8112和let-7 j可能是诊断AIED的潜在生物标志物。
Circulating microRNAs (miRNAs) have been suggested as non-invasive biomarkers for the diagnosis of several autoimmune diseases. However, to the best of our knowledge, no studies have yet examined the miRNA expression profiles in autoimmune inner ear disease (AIED). The present study aimed to use an miRNA sequencing assay to detect the miRNA expression profiles of serum samples from 3 control mice and 3 antigen-induced AIED model mice. Differentially expressed miRNAs (DE-miRNAs) were screened using a t-test. miRNA target prediction was performed using TargetScan Mouse. Then, the miRNA-target gene interaction network was constructed and visualized using Cytoscape software. The underlying functions of the target genes of the DE-miRNAs were predicted using the clusterProfiler package. As a result, 22 miRNAs were identified as DE-miRNAs between AIED and control mice, including 10 upregulated and 12 downregulated genes. Based on the TargetScan Mouse prediction, 1,958 genes were identified as the targets for the 22 DE-miRNAs. Functional analysis indicated that only the target genes of 8 miRNAs were respectively enriched for Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways, among which miR-10b-3p, let-7j and miR-8112 were shared between the two pathway analyses. These 3 miRNAs may be involved in AIED by affecting inflammatory chemokine (miR-10b-3p-C-C motif chemokine 12), Wnt signaling (miR-8112-Wnt9b/Wnt 3a/Wnt2b) and Mucin type O-glycan biosynthesis pathways (let-7j-Galnt2/Galnt12). In conclusion, miR-10b-3p, miR-8112 and let-7j may be underlying biomarkers for diagnosing AIED.
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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