Direct assessment of the rate of chromosomal abnormalities in grade IV human embryos produced by in-vitro fertilization procedure.
Direct assessment of the rate of chromosomal abnormalities in grade IV human embryos produced by in-vitro fertilization procedure.
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DOI：
10.1093/oxfordjournals.humrep.a138497
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发表时间：
1994-02
影响因子：
6.1
通讯作者：
Franck Pellestor;Marie-Catherine Dufour;F. Arnal;Claude Humeau
中科院分区：
文献类型：
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作者：
Franck Pellestor;Marie-Catherine Dufour;F. Arnal;Claude Humeau
在体外受精（IVF）过程中，人类着床前胚胎根据其在光学显微镜下的形态外观分为四个等级。IV级组包括质量差的胚胎。在我们的体外受精项目中，这些胚胎从未被移植或冷冻，因此可用于细胞遗传学分析。对327对参加体外受精计划的夫妇的411个IV级胚胎进行了细胞遗传学分析。总共118个胚胎成功地利用至少一个中期进行核型。正常二倍体染色体仅在12个胚胎中发现，共包含19个中期。其余（90%）均显示异常或异常的染色体补体；非整倍体48例，单染色单体6例；14个胚胎（11.8%）含有单倍体补体，其余44个胚胎呈现嵌合体（2n/3n、n/2n、n/3n）或染色体片段化。此外，还观察到一些结构畸变和重排。这些结果表明，绝大多数IV级人类胚胎存在染色体异常。这证实了这些胚胎质量差的形态学评估，并证明了IV级胚胎的移植和冷冻保存的无用性。
During in-vitro fertilization (IVF) procedures, human preimplantation embryos were classified into four grades according to their morphological appearance under light microscopy. The grade IV group included poor quality embryos. In our IVF programme, these embryos were never transferred or frozen, and were thus available for cytogenetic analysis. Cytogenetic analysis was performed on 411 grade IV embryos from 327 couples participating in the IVF programme. A total of 118 embryos were successfully karyotyped using at least one metaphase. Normal diploid chromosomes were found in only 12 embryos, containing a total of 19 metaphases. All others (90%) showed abnormal or aberrant chromosome complements; 48 were aneuploid and six cases of single chromatids were noted; 14 embryos (11.8%) contained haploid complements, while the remaining 44 exhibited mosaics (2n/3n, n/2n, n/3n) or fragmented chromosome sets. Also, several structural aberrations and rearrangements were observed. These results indicate that the large majority of grade IV human embryos are chromosomally abnormal. This confirms the morphological assessment of the poor quality of these embryos and demonstrates the uselessness of both the transfer and the cryopreservation of grade IV embryos.