Nature of "Tau" immunoreactivity in normal myonuclei and inclusion body myositis.
Nature of "Tau" immunoreactivity in normal myonuclei and inclusion body myositis.
复制标题
正常肌核和包涵体肌炎中“Tau”免疫反应性的性质。
DOI：
10.1002/mus.21471
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发表时间：
2009
期刊：
影响因子：
3.4
通讯作者：
Greenberg,StevenA
中科院分区：
文献类型：
--
作者：
Salajegheh,Mohammad;Pinkus,JackL;Nazareno,Remedios;Amato,AnthonyA;Parker,KennethC;Greenberg,StevenA
磷酸化tau蛋白的肌浆积累已被广泛认为发生在包涵体肌炎中的肌肉疾病中并促成其发病机制。用抗tau抗体（tau-5、pS422和SMI-31）对20份炎性肌病和10份正常肌肉样本沿着以及一系列其他组织进行染色。使用差异溶解和激光捕获显微切割制备肌浆和肌浆部分，并使用pS422和SMI-31抗体进行免疫印迹。所有三种抗体均在正常和患病肌肉的肌核中表现出抗tau免疫反应性，但在其他组织的细胞核中则没有。Western印迹显示pS422和SMI-31对磷酸化tau预期区域外的核蛋白具有免疫反应性。先前报道的指示IBM肌纤维中磷酸化tau异常积累的抗体与正常肌核反应并识别tau以外的蛋白质。正常肌核含有神经丝H或其他未识别的200 kDa蛋白质，具有类似的磷酸化基序，可解释SMI-31免疫反应性。肌肉神经，2009年
Sarcoplasmic accumulation of phosphorylated‐tau has been widely stated to occur in and contribute to the pathogenesis of muscle disease in inclusion body myositis. Twenty inflammatory myopathy and 10 normal muscle samples along with a range of other tissues were stained with anti‐“tau” antibodies (tau‐5, pS422, and SMI‐31). Myonuclear and sarcoplasmic fractions were prepared using differential solubilization and laser‐capture microdissection, and immunoblots were performed using pS422 and SMI‐31 antibodies. All three antibodies demonstrated anti‐tau immunoreactivity in myonuclei from normal and diseased muscle, but not in nuclei from other tissues. Western blots showed pS422 and SMI‐31 immunoreactivity against nuclear proteins outside the region expected for phosphorylated‐tau. Antibodies previously reported to indicate abnormal accumulation of phosphorylated‐tau in IBM myofibers react to normal myonuclei and recognize proteins other than tau. Normal myonuclei contain neurofilament H or other unidentified 200 kDa proteins with similar phosphorylated motifs accounting for SMI‐31 immunoreactivity. Muscle Nerve, 2009