Rapid Assessment of Surface Markers on Cancer Cells Using Immuno-Magnetic Separation and Multi-frequency Impedance Cytometry for Targeted Therapy
Rapid Assessment of Surface Markers on Cancer Cells Using Immuno-Magnetic Separation and Multi-frequency Impedance Cytometry for Targeted Therapy
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DOI：
10.1038/s41598-020-57540-7
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发表时间：
2020-02-20
影响因子：
4.6
通讯作者：
Javanmard, Mehdi
中科院分区：
文献类型：
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作者：
Lin, Zhongtian;Lin, Siang-Yo;Javanmard, Mehdi
对癌细胞表面标志物的快速定性评估可以允许对患者对各种癌症药物的反应进行即时预测。临床前研究用与有效毒素缀合的“活化”间质蛋白酶的抗体靶向细胞显示出作为多种实体瘤的选择性治疗的前景。在本文中，我们实现了一种新的技术，用于电检测蛋白质的癌细胞表面使用多频微流控阻抗细胞仪。该生物传感器由嵌入PDMS微流体通道的玻璃基底上的两个金微电极组成，用于与癌细胞的免疫磁性分离结合，并且能够基于其各种阻抗和频率响应区分裸磁珠、癌细胞和珠-细胞聚集体。我们证明了基于检测培养的外套细胞表面上的“活化的”间质蛋白酶蛋白的概念验证。
The rapid qualitative assessment of surface markers on cancer cells can allow for point-of-care prediction of patient response to various cancer drugs. Preclinical studies targeting cells with an antibody to "activated" matriptase conjugated to a potent toxin show promise as a selective treatment for a variety of solid tumors. In this paper, we implemented a novel technique for electrical detection of proteins on surfaces of cancer cells using multi-frequency microfluidic impedance cytometry. The biosensor, consists of two gold microelectrodes on a glass substrate embedded in a PDMS microfluidic channel, is used in conjugation with immuno-magnetic separation of cancer cells, and is capable of differentiating between bare magnetic beads, cancer cells and bead-cell aggregates based on their various impedance and frequency responses. We demonstrated proof-of-concept based on detection of "activated" matriptase proteins on the surface of cultured Mantle cells.