Identification of inorganic and organic species of phosphorus and its bio-availability in nitrifying aerobic granular sludge
Identification of inorganic and organic species of phosphorus and its bio-availability in nitrifying aerobic granular sludge
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DOI：
10.1016/j.watres.2014.09.054
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发表时间：
2015-01-01
期刊：
影响因子：
12.8
通讯作者：
Lee, Duu-Jong
中科院分区：
文献类型：
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作者：
Huang, Wenhi;Cai, Wei;Lee, Duu-Jong
由于不可再生的磷资源日益枯竭，从污泥中回收磷是环境和社会可持续发展的必要条件。好氧颗粒污泥是一种很有前途的污水处理生物技术，它可以在同步硝化和反硝化过程中形成富磷颗粒污泥。本研究旨在揭示好氧颗粒污泥中无机磷和有机磷形态的变化及其与磷迁移率和生物有效性的关系。采用两个相同的方形反应器培养好氧颗粒污泥，运行120 d，进水氨氮（NH 4-N）在60 d前为100 mg/L，在随后的60 d内增加到200 mg/L（化学需氧量（COD）保持在600 mg/L）。好氧颗粒污泥具有良好的COD去除和硝化效果。结果表明，无机磷（IP）约占TP总量的61.4-67.7%，非磷灰石无机磷（NAIP）约占IP总量的61.9-70.2%。颗粒中NAIP和磷灰石P（AP）的富集量与颗粒中Fe和Ca的含量呈强正相关。X射线衍射（XRD）分析和溶解指数计算表明，颗粒中的主要磷矿物为羟基磷灰石（Ca-5（PO 4）（3）（OH））和磷酸铁（Fe-7（PO 4）（6））。在120天的运行中，好氧颗粒污泥中有机磷（OP）含量维持在7.5 mg/g生物量左右。通过磷-31核磁共振（P-31 NMR）鉴定磷酸单酯（提取物中TP的21.8%）、磷酸二酯（1.8%）和膦酸酯（0.1%）为OP物种。颗粒污泥中NAIP + OP占TP的比例约为80%，说明硝化好氧颗粒污泥中储存了高潜在移动的和生物可利用的P。研究结果为了解好氧颗粒污泥中磷的形态特征提供了新的思路，为污水生物处理中磷的去除和回收技术的发展提供了理论依据。(C)2014爱思唯尔有限公司版权所有。
Phosphorus (P) recovery from sewage sludge is necessary for a sustainable development of the environment and thus the society due to gradual depletion of non-renewable P resources. Aerobic granular sludge is a promising biotechnology for wastewater treatment, which could achieve P-rich granules during simultaneous nitrification and denitriftation processes. This study aimed to disclose the changes in inorganic and organic P species and their correlation with P mobility and bio-availability in aerobic granules. Two identical square reactors were used to cultivate aerobic granules, which were operated for 120 days with influent ammonia nitrogen (NH4-N) of 100 mg/L before day 60 and then increased to 200 mg/L during the subsequent 60 days (chemical oxygen demand (COD) was kept constant at 600 mg/L). The aerobic granules exhibited excellent COD removal and nitrification efficiency. Results showed that inorganic P (IP) was about 61.4-67.7% of total P (TP) and non-apatite inorganic P (NAIP) occupied 61.9-70.2% of IP in the granules. The enrichment amount of NAIP and apatite P (AP) in the granules had strongly positive relationship with the contents of metal ions, i.e. Fe and Ca, respectively accumulated in the granules. X-ray diffraction (XRD) analysis and solution index calculation demonstrated that hydroxyapatite (Ca-5(PO4)(3)(OH)) and iron phosphate (Fe-7(PO4)(6)) were the major P minerals in the granules. Organic P (OP) content maintained around 7.5 mg per gram of biomass in the aerobic granules during the 120 days' operation. Monoester phosphate (21.8% of TP in extract), diester phosphate (1.8%) and phosphonate (0.1%) were identified as OP species by Phosphorus-31 nuclear magnetic resonance (P-31 NMR). The proportion of NAIP + OP to TP was about 80% in the granules, implying high potentially mobile and bio-available P was stored in the nitrifying aerobic granules. The present results provide a new insight into the characteristics of P species in aerobic granules, which could be helpful for developing P removal and recovery techniques through biological wastewater treatment. (C) 2014 Elsevier Ltd. All rights reserved.