Upregulation of ROCK2 in gastric cancer cell promotes tumor cell proliferation, metastasis and invasion
Upregulation of ROCK2 in gastric cancer cell promotes tumor cell proliferation, metastasis and invasion
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DOI：
10.1007/s10238-016-0444-z
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发表时间：
2017-11-01
影响因子：
4.6
通讯作者：
Huang, Hua
中科院分区：
文献类型：
--
作者：
Li, Manhua;Ke, Jing;Huang, Hua
Rho 相关卷曲螺旋蛋白激酶 2 (ROCK2) 被认为是小 GTP 酶 Rho 的效应子，在肿瘤进展和转移中发挥重要作用。然而，ROCK2 在胃癌 (GC) 中的作用尚未确定。本研究表明，与癌旁非癌组织相比，临床GC组织中ROCK2表达显着升高。免疫组织化学分析显示，ROCK2的高表达与肿瘤分级、肿瘤淋巴结转移分期、浸润深度、淋巴结侵犯和Ki-67相关，并在135例胃癌标本中预测不良预后。此外，我们通过细胞计数试剂盒8（CCK-8）实验、集落形成实验、流式细胞术分析和跨孔实验发现，ROCK2上调可促进GC细胞的增殖、转移和侵袭，而ROCK2敲低则导致相反的结果。我们的研究结果支持 ROCK2 是 GC 进展中的重要蛋白质，并将为人类 GC 提供一种新颖、有前景的治疗策略。
Rho-associated coiled-coil-containing protein kinase 2 (ROCK2) has been known as an effector for the small GTPase Rho and plays an important role in tumor progression and metastasis. However, the effect of ROCK2 in gastric cancer (GC) has not been identified. This study showed that ROCK2 expression significantly increased in clinical GC tissues compared with adjacent non-cancer tissues. Immunohistochemistrical analysis showed that high expression of ROCK2 was correlated with tumor grade, tumor-node-metastasis stage, infiltration depth, lymph node invasion and Ki-67, and predicted poor prognosis in 135 gastric cancer specimens. In addition, we found that upregulated ROCK2 promoted proliferation, metastasis and invasion of GC cells, while ROCK2 knockdown led to the opposite results in vitro by Cell Counting Kit-8 (CCK-8) assay, colony formation assays, flow cytometric analysis and trans-well assays. Our findings supported that ROCK2 was a significant protein in the progress of GC and would provide a novel promising therapeutic strategy against human GC.