Altered P-selectin and CD44 expression in the renal tissues and peripheral blood of children with IgA nephropathy
Altered P-selectin and CD44 expression in the renal tissues and peripheral blood of children with IgA nephropathy
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DOI：
10.1007/s11255-008-9512-y
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发表时间：
2009-01
影响因子：
2
通讯作者：
Qiaoling Zhang;Xiaoyun Jiang;Wu Wei;Shuhong Dong;Peng Yaqin;Xiaoqing Guan
中科院分区：
文献类型：
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作者：
Qiaoling Zhang;Xiaoyun Jiang;Wu Wei;Shuhong Dong;Peng Yaqin;Xiaoqing Guan
目的探讨P-选择素（CD 62 P）和CD 44在伊加肾病（IgAN）患儿肾病过程中介导免疫炎症反应的作用，探讨CD 62 P和CD 44在IgAN患儿外周血和肾组织中的协同表达及其与组织病理学、血清学、材料与方法46例IgAN患儿根据病理分级和临床表现分为三组。15例正常儿童血液样本和4例正常肾活检标本作为对照。采用双抗体夹心免疫放射法检测血浆CD 62 P水平;采用ELISA法检测血清CD 44水平。结果IgAN患儿肾组织和外周血中CD 62 P和CD 44协同表达。CD 62 P和CD 44表达的改变不仅与血尿、蛋白尿、血清胆固醇、白蛋白、尿NAG和β2-MG有关，而且与IgAN患儿肾小管间质损伤程度有关。CD 62 P和CD 44在肾组织和外周血中的协同表达，结合血清学和尿液预测因子，可能对儿童IgAN进展的诊断具有重要意义。
ObjectiveTo understand the role of P-selectin (CD62P) and CD44 in mediating immune inflammation in the nephrotic process of children with IgA nephropathy (IgAN), cooperative expression of CD62P and CD44 in peripheral blood and renal tissues of IgAN children was investigated and its association with changes of histopathologic, serologic, and urinary properties was tested.Material and methodsForty-six IgAN children were divided into three groups according to pathologic grades and clinical features. Fifteen blood samples from normal children and four normal renal biopsy specimens were used as controls. Plasma level of CD62P was detected by double antibody sandwich immunoradiometric assay; ELISA was used to determine serum level of CD44. Expression of CD62P and CD44 in renal tissues was determined by immunohistochemistry.ResultsCooperative expression of CD62P and CD44 was detected in renal tissues and peripheral blood of IgAN children. Altered expression of CD62P and CD44 in peripheral blood significantly correlated not only with hematuria, proteinuria, serum cholesterol, and albumin, and with urine NAG and β2-MG, but also with degree of tubulointerstitial injury in IgAN children.ConclusionThe evidence supported CD62P and CD44 as initial and promoting factors mediating immune inflammation in the nephrotic process in IgAN children. The cooperative expression profiles of CD62P and CD44 in renal tissues and peripheral blood combined with serologic and urinary predictors may be important in diagnosis of progression in children with IgAN.