Gastric leptin and Helicobacter pylori infection
Gastric leptin and Helicobacter pylori infection
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作者：
Azuma, T;Suto, H;Kato, T
背景-瘦素调节进食行为，因此可能是与急性和慢性炎症相关的厌食症的介质。最近，在胃上皮中发现了瘦素mRNA和瘦素蛋白。目的-本研究的目的是检测幽门螺杆菌感染对胃瘦素表达的影响，探讨胃瘦素的病理生理作用。方法-对手术切除的人胃组织进行免疫组化和逆转录聚合酶链反应（RT-PCR）检查人胃上皮中瘦素的存在。共有201例H pylori阳性慢性胃炎患者接受了H pylori根除治疗，并从体重指数（BMI）和血清瘦素水平检测感染治愈效果。 201 名患者中的 40 名患者在根除治疗前和根除治疗后三个月采集了胃底粘膜活检标本。对这些样本进行定量RT-PCR，检测根除治疗对胃底粘膜瘦素表达的影响。结果-在胃底腺下半部检测到瘦素免疫反应细胞，在胃底粘膜中也发现了瘦素PCR产物。幽门螺杆菌感染显着增加胃瘦素表达。此外，幽门螺杆菌感染的治愈显着降低了胃瘦素的表达，伴随着体重指数的增加。相比之下，幽门螺杆菌感染治愈后血清瘦素水平没有明显变化。结论——瘦素存在于人胃粘膜中。根除幽门螺杆菌感染后，胃瘦素可能在体重增加中发挥作用。胃瘦素可能具有局部作用而不是全身作用。
Background-Leptin regulates feeding behaviour and therefore may be a mediator of anorexia associated with acute and chronic inflammation. Recently, leptin mRNA and leptin protein were found in the gastric epithelium.Aim-The aim of the present study was to examine the effect of Helicobacter pylori infection on gastric leptin expression to investigate the pathophysiological role of gastric leptin.Methods-Surgically resected human stomach tissues were subjected to immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR) to check for the presence of leptin in the human gastric epithelium. A total of 201 H pylori positive patients with chronic gastritis underwent eradication therapy for H pylori and were examined for the effect of infection cure in terms of body mass index (BMI) and serum leptin levels. Biopsy specimens from the gastric fundic mucosa were obtained from 40 of the 201 patients before and three months after eradication therapy. These samples were subjected to quantitative RT-PCR to examine the effect of eradication therapy on leptin expression in the gastric fundic mucosa.Results-Leptin immunoreactive cells were detected in the lower half of the gastric fundic glands and a leptin PCR product was also found in the gastric fundic mucosa. H pylori infection significantly increased gastric leptin expression. In addition, cure of H pylori infection significantly reduced gastric leptin expression, with a concomitant increase in BMI. In contrast, serum leptin levels did not change significantly after cure of H pylori infection.Conclusion-Leptin is present in the human gastric mucosa. Gastric leptin may play a role in weight gain after eradication of H pylori infection. Gastric leptin may have a local rather than systemic action.