Cpvma1, a Vacuolar H -ATPase Catalytic Subunit of Cryphonectria parasitica, is Essential for Virulence and Hypovirus RNA Accumulation
Cpvma1, a Vacuolar H -ATPase Catalytic Subunit of Cryphonectria parasitica, is Essential for Virulence and Hypovirus RNA Accumulation
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Cpvma1 是 Cryphonectria parasitica 的液泡 H -ATP 酶催化亚基，对于毒力和低病毒 RNA 积累至关重要
DOI：
10.1094/phyto-08-18-0289-r
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发表时间：
2019
期刊：
影响因子：
3.2
通讯作者：
Chen Baoshan
中科院分区：
文献类型：
--
作者：
Li Ru;Bai Shan;He Yuanyuan;Chen Qi;Yao Yanping;Wang Jinzi;Chen Baoshan
液泡H+- atp酶（v - atp酶）是一种保守的atp依赖质子泵，可使真核细胞内的细胞室酸化。通过产生过表达和沉默的Cpvma1菌株，研究了V-ATPase催化亚基Cpvma1的作用。对突变株的表型特征、v - atp酶活性、对培养基中pH和Ca2+升高的反应、对板栗的毒力和细胞中低病毒RNA的积累进行了评估。与野生型菌株相比，cpvma1过表达菌株在表型上无显著差异；然而，cpvma1沉默菌株表现出生长速率降低、产孢量降低、v - atp酶活性和毒力显著降低的表型。此外，Cpvma1的沉默增加了对pH和Ca2+升高的敏感性，暗示Cpvma1在pH适应和Ca2+稳态中起重要作用。此外，cpvma1的沉默导致低病毒RNA的积累显著减少。综上所述，我们的研究结果表明，Cpvma1在低病毒寄生的表型性状和毒力以及RNA积累的调控中起着重要作用。
The vacuolar H+-ATPases (V-ATPases) are conserved ATP-dependent proton pumps that acidify intracellular compartments in eukaryotic cells. The role of Cpvma1, a V-ATPase catalytic subunit A ofCryphonectria parasitica, was investigated by generatingcpvma1-overexpressing andcpvma1-silenced strains. The mutant strains were evaluated for phenotypic characteristics, V-ATPase activity, response to elevated pH and Ca2+in the medium, virulence on chestnut, and accumulation of hypovirus RNA in the cells. Compared with the wild-type strain,cpvma1-overexpressing strains showed no significant difference in phenotype; however,cpvma1-silenced strains exhibited a phenotype of reduced growth rate, lower level of sporulation, and a marked decrease in V-ATPase activity and virulence. In addition, silencing ofcpvma1increased sensitivity to elevated pH and Ca2+, implicating an important role for Cpvma1 in pH adaptation and Ca2+homeostasis. Furthermore, silencing ofcpvma1resulted in significantly decreased accumulation of hypoviral RNA. Taken together, our results indicate that Cpvma1 plays an important role in the regulation of phenotypic traits and virulence and the accumulation of hypovirus RNA inC. parasitica.