Age-related changes in the collagen network and toughness of bone
Age-related changes in the collagen network and toughness of bone
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DOI：
10.1016/s8756-3282(01)00697-4
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发表时间：
2002-07-01
期刊：
影响因子：
4.1
通讯作者：
Agrawal, CM
中科院分区：
文献类型：
--
作者：
Wang, X;Shen, X;Agrawal, CM
这项研究的假设是，骨骼中胶原蛋白网络的机械完整性随着年龄的增长而恶化，这种不利的变化与老化骨骼的韧性下降有关。为了验证这一假设，对来自 19 岁至 89 岁捐献者的 30 具人类尸体股骨进行了测试，以确定脱矿骨和新鲜骨样本的机械性能与年龄相关的变化。除了骨孔隙率、骨密度以及矿物相和有机相的重量分数之外，还确定了这些骨样本的胶原变性和胶原交联浓度（HP，羟赖氨吡啶啉；LP，赖氨吡啶啉；PE，戊糖苷）作为函数年龄。方差分析（ANOVA）表明，年龄相关的变化反映在骨强度、断裂功和断裂韧性的下降上；强度、弹性模量下降，并导致胶原网络断裂；以及戊糖素（非酶糖化标记物）浓度增加和骨孔隙率增加。测量参数的回归分析表明，与年龄相关的骨折（尤其是屈服后部分）工作量的减少与胶原网络机械完整性的恶化显着相关。这项研究的结果表明，随着人们年龄的增长，胶原蛋白网络会发生不利的变化，这种变化可能导致骨骼韧性下降。此外，结果表明，非酶糖化可能是导致胶原蛋白变化的重要因素，从而导致与年龄相关的骨质量恶化。
The hypothesis of this study is that the mechanical integrity of the collagen network in bone deteriorates with age, and such adverse changes correlate with the decreased toughness of aged bone. To test the hypothesis, 30 human cadaveric femurs from donors ranging from 19 to 89 years of age were tested to determine the age-related changes in the mechanical properties of demineralized bone and fresh bone samples. Along with bone porosity, bone density, and weight fractions of the mineral and organic phases, collagen denaturation and concentrations of collagen cross-links (HP, hydroxylysylpyridinoline; LP, lysylpyridinoline; PE, pentosidine) were determined for these bone specimens as a function age. Analysis of variance (ANOVA) showed that age-dependent changes were reflected in the decreased strength, work to fracture, and fracture toughness of bone; in the decreased strength, elastic modulus, and work to fracture of the collagen network; as well as in the increased concentration of pentosidine (a marker of nonenzymatic glycation) and increased bone porosity. Regression analyses of the measured parameters showed that the age-related decrease in work to fracture of bone (especially its postyield portion) correlated significantly with deterioration in the mechanical integrity of the collagen network. The results of this study indicate that the adverse changes in the collagen network occur as people age and such changes may lead to the decreased toughness of bone. Also, the results suggest that nonenzymatic glycation may be an important contributing factor causing changes in collagen and, consequently, leading to the age-related deterioration of bone quality.