Involvement of trigeminal transition zone and laminated subnucleus caudalis in masseter muscle hypersensitivity associated with tooth inflammation.
Involvement of trigeminal transition zone and laminated subnucleus caudalis in masseter muscle hypersensitivity associated with tooth inflammation.
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DOI：
10.1371/journal.pone.0109168
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Iwata K
中科院分区：
文献类型：
--
作者：
Shimizu K;Matsumoto K;Noma N;Matsuura S;Ohara K;Komiya H;Watase T;Ogiso B;Tsuboi Y;Shinoda M;Hatori K;Nakaya Y;Iwata K
采用大鼠牙髓炎/根尖周炎模型，研究咬肌反应域外增强与牙齿炎症相关的机制。根尖周骨丢失逐渐增加，在上磨牙牙髓完全应用弗氏佐剂(CFA)后6周达到高峰。3d时，M1CFA组大鼠三叉神经内侧亚核/尾侧移行区(Vi/Vc)的Fos免疫反应阳性(IR)细胞数明显多于M1VIH组大鼠。M1CFA加咬肌(M1CFA/MASS)辣椒素(M1CFA/MASS CAP)大鼠的Fos-IR细胞数明显多于对侧Vc和Vi/Vc的M1VEH/MASS VEH大鼠。M1CFA/质量帽和M1veh/质量帽大鼠Vi/Vc的磷酸化细胞外信号调节激酶(PERK)-IR细胞数明显多于使用M1Vehicle或CFA的M1Vi/Vc组大鼠。CFA给药后6周，Vi/Vc的Fos-IR细胞数显著增加，而Vc内Fos-IR细胞数无明显变化。CFA给药后，辣椒素给药组大鼠的PERK-IR细胞数明显多于给药组。然而，辣椒素对CFA应用大鼠牙髓Vi/Vc的Fos-IR细胞数量没有进一步的影响。大剂量辣椒素作用后大鼠二腹肌电(d-EMG)活动在CFA给药后第3天显著增加，并持续到第6周，这种增加和持续时间显著减弱。PD98059，一种MEK1抑制剂。这些结果提示，Vi/Vc和Vc神经元兴奋参与了根尖周炎或急性牙髓炎引起的肿块的域外痛敏反应。此外，Vi/Vc和Vc中ERK的磷酸化在牙髓炎或根尖周炎后咬肌痛过敏中起关键作用。
A rat model of pulpitis/periapical periodontitis was used to study mechanisms underlying extraterritorial enhancement of masseter response associated with tooth inflammation. Periapical bone loss gradually increased and peaked at 6 weeks after complete Freund’s adjuvant (CFA) application to the upper molar tooth pulp (M1). On day 3, the number of Fos-immunoreactive (IR) cells was significantly larger in M1 CFA rats compared with M1 vehicle (veh) rats in the trigeminal subnucleus interpolaris/caudalis transition zone (Vi/Vc). The number of Fos-IR cells was significantly larger in M1 CFA and masseter (Mass) capsaicin applied (M1 CFA/Mass cap) rats compared with M1 veh/Mass veh rats in the contralateral Vc and Vi/Vc. The number of phosphorylated extracellular signal-regulated kinase (pERK)-IR cells was significantly larger in M1 CFA/Mass cap and M1 veh/Mass cap rats compared to Mass-vehicle applied rats with M1 vehicle or CFA in the Vi/Vc. Pulpal CFA application caused significant increase in the number of Fos-IR cells in the Vi/Vc but not Vc on week 6. The number of pERK-IR cells was significantly lager in the rats with capsaicin application to the Mass compared to Mass-vehicle treated rats after pulpal CFA- or vehicle-application. However, capsaicin application to the Mass did not further affect the number of Fos-IR cells in the Vi/Vc in pulpal CFA-applied rats. The digastric electromyographic (d-EMG) activity after Mass-capsaicin application was significantly increased on day 3 and lasted longer at 6 weeks after pulpal CFA application, and these increase and duration were significantly attenuated by i.t. PD98059, a MEK1 inhibitor. These findings suggest that Vi/Vc and Vc neuronal excitation is involved in the facilitation of extraterritorial hyperalgesia for Mass primed with periapical periodontitis or acute pulpal-inflammation. Furthermore, phosphorylation of ERK in the Vi/Vc and Vc play pivotal roles in masseter hyperalgesia after pulpitis or periapical periodontitis.
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