Human endothelial cells in culture produce platelet-activating factor (1-alkyl-2-acetyl-sn-glycero-3-phosphocholine) when stimulated with thrombin.
Human endothelial cells in culture produce platelet-activating factor (1-alkyl-2-acetyl-sn-glycero-3-phosphocholine) when stimulated with thrombin.
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当用凝血酶刺激时，培养的人内皮细胞会产生血小板激活因子（1-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱）。
DOI：
10.1073/pnas.81.11.3534
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发表时间：
1984
影响因子：
11.1
通讯作者：
T. McIntyre
中科院分区：
文献类型：
--
作者：
S. Prescott;G. Zimmerman;T. McIntyre
当用人凝血酶刺激时，培养的人内皮细胞会产生血小板激活因子（1-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱；PAF）。对凝血酶的反应具有剂量依赖性，半最大效应为 0.17 单位/ml。通过放射性标记前体的掺入、其在色谱系统中的行为、生物活性的恢复以及磷脂酶 A2 处理的效果，将该产物鉴定为 PAF。 [3H]乙酸盐在 PAF 中的掺入量在 5 分钟时达到最大，此后逐渐减少。根据中性粒细胞聚集活性的量来判断，在凝血酶暴露 5 分钟内，内皮细胞每 10(6) 个细胞产生 0.10-0.17 nmol PAF。这种内皮细胞对血小板活化和中性粒细胞趋化作用的有效激动剂的产生表明，它可能在维持血管完整性中发挥作用，并且可能在血栓形成和动脉粥样硬化等病理事件中发挥作用。
Cultured human endothelial cells produce platelet-activating factor (1-alkyl-2-acetyl-sn-glycero-3-phosphocholine; PAF) when stimulated with human thrombin. The response to thrombin is dose dependent, with a half-maximal effect at 0.17 unit/ml. The product is identified as PAF by the incorporation of radiolabeled precursors, its behavior in chromatographic systems, the recovery of biological activity, and the effect of treatment with phospholipase A2. Incorporation of [3H]acetate into PAF is maximal by 5 min and decreases thereafter. Endothelial cells produce 0.10-0.17 nmol of PAF per 10(6) cells in a 5-min exposure to thrombin, as judged by the amount of neutrophil-aggregating activity. The production of this potent agonist for platelet activation and neutrophil chemotaxis by endothelial cells suggests that it may play a role in the maintenance of vascular integrity and perhaps in pathological events such as thrombosis and atherogenesis.