Nucleated cells circulating in the peripheral blood contribute to the repair of osteochondral defects only in the early phase of healing
Nucleated cells circulating in the peripheral blood contribute to the repair of osteochondral defects only in the early phase of healing
复制标题
外周血中循环的有核细胞仅在愈合的早期阶段有助于骨软骨缺损的修复
DOI：
10.1002/term.1536
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Nakamura H.
中科院分区：
文献类型：
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作者：
Okano T.;Wakitani S.;Okabe T.;Takahashi M.;Koike T.;Nakamura H.
在绿色荧光蛋白（GFP）转基因和野生大鼠联体共生模型中评估了外周血循环细胞参与骨软骨缺损自然修复过程的作用。联体共生手术两周后，通过流式细胞术分析证实了联体大鼠之间的血管连通。在每只大鼠股骨的髌骨沟内制作直径1.5 mm、深度1.0 mm的骨软骨缺损。手术后1、2、4和24周进行组织学检查。在术后早期（1-4周），两只共生大鼠的缺陷处均存在 GFP 阴性和阳性细胞。在野生共生大鼠的修复组织中部分证实了 GFP 阳性软骨细胞。在术后后期（24周），修复的缺损被来自邻近组织而不是来自外周血的细胞占据。术后1周修复组织中源自外周血的细胞比例约为30-40%，24周时降至0-7%。这些结果证实，外周血中循环的细胞有助于骨软骨缺损的修复，特别是在愈合的早期阶段。因此，外周血不仅提供修复所需的因子，还提供参与伤口愈合过程的细胞群。版权所有 © 2012 约翰·威利父子有限公司
The role of cells circulating in the peripheral blood to participate in the natural repair process of osteochondral defects was evaluated in a green fluorescent protein (GFP) transgenic and wild rat parabiosis model. Two weeks after the parabiosis operation, vascular communication between the conjoined rats was confirmed by flow‐cytometry analysis. A 1.5 mm diameter and 1.0 mm depth osteochondral defect was made in the patellar groove of each rat femoral bone. Histological examination was performed at 1, 2, 4 and 24 weeks following surgery. In the early postoperative phase (1–4 weeks) there were GFP‐negative and ‐positive cells in the defects of both parabiotic rats. GFP‐positive chondrocytes were confirmed partly in the repair tissue of the wild parabiotic rat. In the late postoperative phase (24 weeks), the repaired defects were occupied by cells originating from the adjacent tissue and not from the peripheral blood. The ratio of cells originating from the peripheral blood was approximately 30–40% in the repair tissue at 1 week after surgery, reduced to 0–7% at 24 weeks. From these results it is confirmed that cells circulating in the peripheral blood contributed to the repair of the osteochondral defects, particularly in the early phase of healing. Thus, peripheral blood not only supplies the factors needed for repair but also provides a cell population involved in the wound‐healing process. Copyright © 2012 John Wiley & Sons, Ltd.