mefE is necessary for the erythromycin-resistant M phenotype in Streptococcus pneumoniae
mefE is necessary for the erythromycin-resistant M phenotype in Streptococcus pneumoniae
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mefE 对于肺炎链球菌中红霉素耐药 M 表型是必需的
DOI：
10.1128/aac.41.10.2251
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发表时间：
1997
影响因子：
4.9
通讯作者：
J. Sutcliffe
中科院分区：
文献类型：
--
作者：
A. Tait;J. Clancy;Melissa Cronan;Fadia B. Dib;L. Wondrack;Wei Yuan;J. Sutcliffe
最近，研究表明，相当数量的红霉素耐药肺炎链球菌和化脓链球菌菌株含有一个决定簇，通过推定的外排泵介导耐药性。从三株链球菌中克隆了编码红霉素抗性决定簇的基因，并进行了序列测定。携带M表型的肺炎（大环内酯类耐药，但克林霉素和链阳菌素B敏感）。mefE的DNA序列几乎相同，任何两个菌株的基因之间只有2个核苷酸的差异。将mefE序列与S.在化脓性链球菌中，发现这两个基因密切相关（90%同一性）。链球菌菌株pneumoniae的构建以确认mefE是赋予红霉素抗性所必需的，并探索泵的底物特异性;没有鉴定出14-和15-元大环内酯以外的底物。
Recently, it was shown that a significant number of erythromycin-resistant Streptococcus pneumoniae and Streptococcus pyogenes strains contain a determinant that mediates resistance via a putative efflux pump. The gene encoding the erythromycin-resistant determinant was cloned and sequenced from three strains of S. pneumoniae bearing the M phenotype (macrolide resistant but clindamycin and streptogramin B susceptible). The DNA sequences of mefE were nearly identical, with only 2-nucleotide differences between genes from any two strains. When the mefE sequences were compared to the mefA sequence from S. pyogenes, the two genes were found to be closely related (90% identity). Strains of S. pneumoniae were constructed to confirm that mefE is necessary to confer erythromycin resistance and to explore the substrate specificity of the pump; no substrates other than 14- and 15-membered macrolides were identified.
人工转座子在体外有效整合到质粒靶标中：DNA 作图、测序和遗传分析的有用工具。
影响因子：
14.9
作者：
Devine,SE;Boeke,JD
通讯作者：
Boeke,JD
影响因子：
56.9
作者：
NIKAIDO, H
通讯作者：
NIKAIDO, H