Microarray Analysis in Glioblastomas.
Microarray Analysis in Glioblastomas.
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DOI：
10.1007/7651_2015_245
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Aghi, Manish K
中科院分区：
文献类型：
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作者：
Bhawe, Kaumudi M;Aghi, Manish K
为了将胶质母细胞瘤细胞系或肿瘤的基因表达特征与对化疗的响应相关联（DeLay et al.，Clin Cancer Res 18：2930-2942，2012）为了将基因表达模式与胶质母细胞瘤细胞的生物学特征如增殖或侵袭性相关联（Jiang等人，PLoS One 8：e66008，2013）为了发现基于基因表达特征的新的肿瘤分类系统，并将治疗反应和预后与这些特征相关联（Huse等人，Annu Rev Med 64：59-70，2013; Verhaak等人，Cancer Cell 17：98-110，2010）使用细胞系或患者样品作为起始材料进行胶质母细胞瘤中的微阵列分析。当前文献的调查指出，基于转录的微阵列和基于免疫组织化学（IHC）的组织微阵列是神经源性癌症的首选方法。微阵列分析可以用于各种目的，包括以下：虽然研究人员有时可以使用从NCBI基因表达综合数据库（英语：NCBI Gene Expression Omnibus）获得的存档肿瘤基因表达数据来回答他们的问题，但通常必须运行新的阵列才能充分回答特定的问题。在这里，我们提供了一个详细的描述微阵列方法，如何选择适当的方法，为一个给定的问题，和分析策略，可以使用。蛋白质微阵列的实验方法超出了本章的范围，但基于转录的微阵列的基本样品制备技术包括在这里。
To correlate gene expression signatures of glioblastoma cell lines or tumors with response to chemotherapy (DeLay et al., Clin Cancer Res 18:2930–2942, 2012) To correlate gene expression patterns with biological features like proliferation or invasiveness of the glioblastoma cells (Jiang et al., PLoS One 8:e66008, 2013) To discover new tumor classificatory systems based on gene expression signature, and to correlate therapeutic response and prognosis with these signatures (Huse et al., Annu Rev Med 64:59–70, 2013; Verhaak et al., Cancer Cell 17:98–110, 2010) Microarray analysis in glioblastomas is done using either cell lines or patient samples as starting material. A survey of the current literature points to transcript-based microarrays and immunohistochemistry (IHC)-based tissue microarrays as being the preferred methods of choice in cancers of neurological origin. Microarray analysis may be carried out for various purposes including the following: While investigators can sometimes use archived tumor gene expression data available from repositories such as the NCBI Gene Expression Omnibus to answer their questions, new arrays must often be run to adequately answer specific questions. Here, we provide a detailed description of microarray methodologies, how to select the appropriate methodology for a given question, and analytical strategies that can be used. Experimental methodology for protein microarrays is outside the scope of this chapter, but basic sample preparation techniques for transcript-based microarrays are included here.