Complex formation of NS5B with NS3 and NS4A proteins of hepatitis C virus
Complex formation of NS5B with NS3 and NS4A proteins of hepatitis C virus
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DOI：
10.1006/bbrc.1998.8202
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发表时间：
1998-03-06
影响因子：
3.1
通讯作者：
Hotta, H
中科院分区：
文献类型：
--
作者：
Ishido, S;Fujita, T;Hotta, H
目前，HCV基因组的复制机制仍不清楚。最近，HCV的NS 5 B和NS 3分别显示出RNA依赖性RNA聚合酶和解旋酶活性，这两者对于病毒RNA复制是不可或缺的。在这项研究中，我们研究了NS 5 B与NS 3和NS 4 A（NS 3的辅因子）的复合物形成。我们在这里表明，NS 5 B通过NS 3的氨基末端部分与NS 3形成复合物。NS 3-NS 5 B复合物的形成在存在和不存在NS 4A的情况下都发生。我们还证明，NS 5 B形成一个复杂的NS 4A在没有NS 3。这些结果表明，NS 3，NS 4A和NS 5 B相互作用，形成一个复合物，作为HCV复制机制的一部分。(C)北京：科学出版社.
At present, the mechanism of replication of the HCV genome remains unclear. Recently, NS5B and NS3 of HCV have been shown to exhibit RNA-dependent RNA polymerase and helicase activities, respectively, both of which are indispensable for virus RNA replication. In this study, we examined the complex formation of NS5B with NS3 and NS4A, a cofactor for NS3. We show here that NS5B forms a complex with NS3 through an amino-terminal portion of NS3. The NS3-NS5B complex formation took place both in the presence and absence of NS4A. We also demonstrate that NS5B form a complex with NS4A in the absence of NS3. These results suggest that NS3, NS4A and NS5B interact with each other to form a complex that functions as part of the replication machinery of HCV. (C) 1998 Academic Press.