Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells
Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells
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DOI：
10.1128/aem.01198-07
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发表时间：
2007-12-01
影响因子：
4.4
通讯作者：
Breidt, F., Jr.
中科院分区：
文献类型：
--
作者：
Pan, Y.;Breidt, F., Jr.
在存在死细胞的情况下，使用单叠氮丙啶（PMA）和单叠氮乙啶对活单核细胞增生李斯特菌细胞进行计数。PMA对L.单核细胞增多症活细胞计数与PMA处理的细胞的实时PCR阈值循环值在4-log范围内线性相关。
Propidium monoazide (PMA) and ethidium monoazide were used for enumeration of viable Listeria monocytogenes cells in the presence of dead cells. PMA had no antimicrobial effect on L. monocytogenes. Viable cell counts were linearly related to real-time PCR threshold cycle values for PMA-treated cells from planktonic and biofilm sources over a 4-log range.