Enhanced proliferation and efficient transmission of Candidatus Liberibacter asiaticus by adult Diaphorina citri after acquisition feeding in the nymphal stage
Enhanced proliferation and efficient transmission of Candidatus Liberibacter asiaticus by adult Diaphorina citri after acquisition feeding in the nymphal stage
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DOI：
10.1111/j.1744-7348.2009.00317.x
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发表时间：
2009-01-01
影响因子：
2.6
通讯作者：
Ashihara, W.
中科院分区：
文献类型：
--
作者：
Inoue, H.;Ohnishi, J.;Ashihara, W.
采用TaqMan实时荧光定量PCR技术对柑橘木虱Diaphorina citri中的黄龙病病原菌亚洲黄龙杆菌进行了定量检测。在木虱作为五龄幼虫或成虫暴露于Ca. L.亚洲烟草感染的植物进行24小时的获取访问期。当成年人吃Ca。L. asiaticus感染植株后，细菌浓度没有显著增加，病原菌没有传播到任何柑橘幼苗。相反，当木虱取食受感染的植物作为寄生虫时，病原体的浓度从最初的收购日分别增加了25倍、360倍和130倍，分别为10、15和20天。此外，病原体被成功地传播到67%的柑橘幼苗新兴的成年人。我们的数据表明，细菌繁殖到木虱中是有效传播的必要条件，并表明成年人很难传播病原体，除非他们以寄生虫的形式获得它。
We carried out a quantitative detection of Candidatus Liberibacter asiaticus, the bacterium associated with the disease of huanglongbing, in the vector psyllid Diaphorina citri by using a TaqMan real-time PCR assay. The concentration of the bacterium was monitored at 5-day intervals for a period of 20 days after psyllids were exposed as fifth instar nymphs or adults to a Ca. L. asiaticus-infected plant for an acquisition access period of 24 h. When adults fed on Ca. L. asiaticus-infected plant, the concentration of the bacterium did not increase significantly and the pathogen was not transmitted to any citrus seedlings. In contrast, when psyllids fed on infected plant as nymphs, the concentration of the pathogen significantly increased by 25-, 360- and 130-fold from the initial acquisition day to 10, 15 and 20 days, respectively. Additionally, the pathogen was successfully transmitted to 67% of citrus seedlings by emerging adults. Our data suggested that multiplication of the bacterium into the psyllids is essential for an efficient transmission and show that it is difficult for adults to transmit the pathogen unless they acquire it as nymphs.