Glucose uptake and glycogen synthesis in muscles from immobilized limbs.
Glucose uptake and glycogen synthesis in muscles from immobilized limbs.
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固定肢体肌肉中的葡萄糖摄取和糖原合成。
DOI：
10.1152/jappl.1984.56.2.431
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Booth,FW
中科院分区：
文献类型：
--
作者：
Nicholson,WF;Watson,PA;Booth,FW
本研究的目的是确定是否在葡萄糖代谢的缺陷，在固定四肢的小鼠肌肉中的胰岛素结合，胰岛素反应性，葡萄糖供应，和胰岛素激活糖原合成酶的改变有关。通过体外方法对这些进行了测试。胰岛素诱导的2-脱氧葡萄糖摄取到小鼠比目鱼肌的最大反应发生在肢体固定的第3和第8小时之间，这表明胰岛素反应性降低。胰岛素与24小时固定肢体肌肉的特异性结合缺乏变化，表明胰岛素受体数量的变化在胰岛素刺激葡萄糖代谢失败中没有发挥作用。它不能刺激来自固定肢体的肌肉中的糖原合成，部分是由于缺乏葡萄糖供应糖原合成，也是由于胰岛素不能有效地增加来自24小时固定肢体的肌肉中的糖原合成酶的活性形式的百分比。
The purposes of this study were to determine whether the defects in glucose metabolism in muscles of immobilized limbs of mice were related to alterations in insulin binding, insulin responsiveness, glucose supply, and insulin activation of glycogen synthase. These were tested by in vitro methodology. A significant lessening in the insulin-induced maximal response of 2-deoxyglucose uptake into the mouse soleus muscle occurred between the 3rd and 8th h of limb immobilization, suggesting a decreased insulin responsiveness. Lack of change in the specific binding of insulin to muscles of 24-h immobilized limbs indicates that a change in insulin receptor number did not play a role in the failure of insulin to stimulate glucose metabolism. It's inability to stimulate glycogen synthesis in muscle from immobilized limbs is due, in part, to a lack of glucose supply to glycogen synthesis and also to the ineffectiveness of insulin to increase the percentage of glycogen synthase in its active form in muscles from 24-h immobilized limbs.