ORGANIZATION AND GEL CONTRACTION BY HUMAN COLONIC-CARCINOMA (HCA-7) SUBLINES GROWN IN 3-DIMENSIONAL COLLAGEN GEL
ORGANIZATION AND GEL CONTRACTION BY HUMAN COLONIC-CARCINOMA (HCA-7) SUBLINES GROWN IN 3-DIMENSIONAL COLLAGEN GEL
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DOI：
10.1002/ijc.2910600626
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发表时间：
1995-03-16
影响因子：
6.4
通讯作者：
PIGNATELLI, M
中科院分区：
文献类型：
--
作者：
KIRKLAND, SC;HENDERSON, K;PIGNATELLI, M
细胞-基质相互作用在控制人结肠癌亚系表型异质性中的作用已被研究。先前从单个人结肠癌细胞系 HCA-7 中分离出的四种细胞系（集落 1、集落 3、集落 6 和集落 30）在 3 维胶原凝胶中生长。在胶原蛋白中，4 个亚系的生长范围从组织良好的腺体结构（集落 30）到细长的分支结构（集落 3）。细胞在胶原中组织成腺体结构的能力与其异种移植物中观察到的分化程度相关。某些亚系，尤其是菌落 3，能够收缩胶原蛋白凝胶。凝胶收缩可以被针对 α(2) 整联蛋白链的功能阻断抗体部分抑制，但不能被针对 α(3) 整联蛋白链的抗体部分抑制，这表明 α(2) 整联蛋白在收缩过程中的作用。此外，用功能阻断性 α(2) 抗体处理的集落 3 培养物形成更紧凑的结构，且生长有限，这表明 α(2) 整合素在细胞迁移中的作用。胶原凝胶中的凝胶收缩和细胞迁移主要限于 I 亚系、集落 3。随后证明 α(2) 整合素参与这两个过程，表明整合素表达和功能在产生这些细胞系表现出的表型异质性中发挥作用。 (C) 1995 Wiley-Liss, Inc.
The role of cell-matrix interactions in controlling phenotypic heterogeneity in human colonic carcinoma sublines has been investigated. Four cell lines (colony 1, colony 3, colony 6 and colony 30) previously isolated from a single human colonic carcinoma cell line, HCA-7, were grown in 3-dimensional collagen gels. In collagen, the growth of the 4 sublines ranged from well-organised glandular structures (colony 30) to elongated branching structures (colony 3). The capability of cells to organise into glandular structures in collagen correlated with the degree of differentiation observed in their xenografts. Certain sublines, most notably colony 3, were able to contract the collagen gel. Gel contraction could be partially inhibited by a function-blocking antibody directed to the alpha(2) integrin chain but not by an antibody directed to the alpha(3) integrin chain demonstrating a role for alpha(2) integrin in the contraction process. In addition, colony 3 cultures treated with the function-blocking alpha(2) antibody formed more compact structures with limited outgrowth, suggesting a role for alpha(2) integrin in cell migration. Gel contraction and cell migration in collagen gel was largely restricted to I subline, colony 3. The subsequent demonstration that alpha(2) integrin is involved in both of these processes suggests that integrin expression and function has a role in generating the phenotypic heterogeneity exhibited by these cell lines. (C) 1995 Wiley-Liss, Inc.