Quantifying Preferences and Responsiveness of Marine Zooplankton to Changing Environmental Conditions using Microfluidics.
Quantifying Preferences and Responsiveness of Marine Zooplankton to Changing Environmental Conditions using Microfluidics.
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DOI：
10.1371/journal.pone.0140553
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Arendt D
中科院分区：
文献类型：
--
作者：
Ramanathan N;Simakov O;Merten CA;Arendt D
全球环境变化显着影响海洋物种组成。然而，迄今为止，分析这些变化对海洋浮游动物群落的影响主要局限于通过死亡率测定来评估致死剂量，因此无法直接评估首选条件或优先条件。在这里，我们使用微流体装置来表征活跃游动的浮游动物的个体行为，并定量确定它们的生态偏好。对于环节动物浮游动物模型 Platynereis dumerilii，我们观察到比桡足类 Euterpina acutifrons 更广泛的 pH 偏好，并揭示了以前未识别的具有不同 pH 偏好的亚群。对于扁沙蚕，引起响应（响应性）所需的最小浓度差为 H+ 约 1 μM，NaCl 约 13.7 mM。此外，利用激光消融，我们发现表达嗅觉素的感觉细胞介导扁沙蚕前肠的化学反应。总而言之，我们的微流体方法可以精确评估和功能性理解环境感知对浮游生物行为的影响。
Global environmental change significantly affects marine species composition. However, analyzing the impact of these changes on marine zooplankton communities was so far mostly limited to assessing lethal doses through mortality assays and hence did not allow a direct assessment of the preferred conditions, or preferendum. Here, we use a microfluidic device to characterize individual behavior of actively swimming zooplankton, and to quantitatively determine their ecological preferendum. For the annelid zooplankton model Platynereis dumerilii we observe a broader pH preferendum than for the copepod Euterpina acutifrons, and reveal previously unrecognized sub-populations with different pH preferenda. For Platynereis, the minimum concentration difference required to elicit a response (responsiveness) is ~1 μM for H+ and ~13.7 mM for NaCl. Furthermore, using laser ablations we show that olfactomedin-expressing sensory cells mediate chemical responsiveness in the Platynereis foregut. Taken together, our microfluidic approach allows precise assessment and functional understanding of environmental perception on planktonic behaviour.