SPECIFICITY OF COMBINATION BETWEEN MUCOPEPTIDE PRECURSORS AND VANCOMYCIN OR RISTOCETIN
SPECIFICITY OF COMBINATION BETWEEN MUCOPEPTIDE PRECURSORS AND VANCOMYCIN OR RISTOCETIN
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DOI：
10.1042/bj1110195
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发表时间：
1969-01-01
影响因子：
4.1
通讯作者：
PERKINS, HR
中科院分区：
文献类型：
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作者：
PERKINS, HR
层析、电泳和差示紫外光谱表明，万古霉素和瑞斯托霉素与不同细菌的粘肽前体形成复合物。存在等摩尔比例的抗生素和肽。研究了反应的特异性，发现反应的最小分子是乙酰基-d-丙氨酰-d-丙氨酸。ThisC-末端二肽序列必须存在于复合物的形成;构型的改变或酯化阻止了它。保留抗生素活性的修饰万古霉素也与适当的肽结合。从差示吸收结果估计更稳定的复合物的解离常数。讨论了复合物形成与抗菌作用的关系。青霉素被认为是酰基-d-丙氨酰-d-丙氨酸的类似物，也改变了万古霉素的光谱;苄基青霉噻唑酸钠也是如此。
Vancomycin and ristocetin formed complexes on being mixed with mucopeptide precursors from various bacteria, as shown by chromatography, electrophoresis and differential ultraviolet spectra. Equimolar proportions of antibiotic and peptide were present. The specificity of the reaction was studied and the smallest molecule found to react was acetyl-d-alanyl-d-alanine. ThisC-terminal dipeptide sequence must be present for complex-formation; change of configuration or esterification prevented it. Modified vancomycins that retained antibiotic activity also combined with appropriate peptides. The dissociation constants of the more stable complexes were estimated from the differential-absorption results. The relationship of complex-formation to antibiotic action is discussed. Penicillin, supposed to be an analogue of acyl-d-alanyl-d-alanine, also modified the spectrum of vancomycin; so, too, did sodium benzylpenicilloate.