Real Time, High Resolution Video Imaging of Apoptosis in Single Cells with a Polymeric Nanoprobe
Real Time, High Resolution Video Imaging of Apoptosis in Single Cells with a Polymeric Nanoprobe
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DOI：
10.1021/bc1004119
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发表时间：
2011-02-01
影响因子：
4.7
通讯作者：
Choi, Kuiwon
中科院分区：
文献类型：
--
作者：
Lee, Seulki;Choi, Ki Young;Choi, Kuiwon
我们报告了一种新的凋亡纳米探针（Apo-NP）的基础上设计的聚合物纳米粒子平台。这种简单的一步技术能够增强活细胞凋亡时的荧光信号，从而实现单细胞和体内凋亡的实时成像。Apo-NP有效地将化学标记的、双淬灭的caspase-3敏感性荧光肽递送到细胞中，允许caspase-3依赖性强荧光放大在凋亡细胞中实时和高分辨率成像。Apo-NP的设计平台是灵活的，并且可以针对广泛的应用进行微调，例如在病理学中鉴定半胱天冬酶相关的细胞凋亡和用于监测细胞凋亡药物在癌症治疗中的治疗功效。
We report a new apoptosis nanoprobe (Apo-NP) designed on the basis of a polymer nanoparticle platform. This simple one-step technique is capable of boosting fluorescence signals upon apoptosis in living cells, enabling real-time imaging of apoptosis in single cells and in vivo. The Apo-NP efficiently delivers chemically labeled, dual-quenched caspase-3-sensitive fluorogenic peptides into cells, allowing caspase-3-dependent strong fluorescence amplification to be imaged in apoptotic cells in real-time and at high resolution. The design platform of the Apo-NP is flexible and can be fine-tuned for a wide array of applications such as identification of caspase-related apoptosis in pathologies and for monitoring therapeutic efficacy of apoptotic drugs in cancer treatment.