Synthesis of magnetic core-shell Fe3O4-Au nanoparticle for biomolecule immobilization and detection
Synthesis of magnetic core-shell Fe3O4-Au nanoparticle for biomolecule immobilization and detection
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DOI：
10.1007/s11051-009-9749-0
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发表时间：
2010-05-01
影响因子：
2.5
通讯作者：
Pekmez, Kadir
中科院分区：
文献类型：
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作者：
Tamer, Ugur;Gundogdu, Yusuf;Pekmez, Kadir
具有适当表面修饰的单分散磁性纳米颗粒的生产在生物医学应用中引起了越来越多的关注，包括药物输送、从基质中分离和纯化生物分子。在本研究中，我们首次报道了在超声处理下使用硼氢化物还原 HAuCl4 在水溶液中快速、室温反应合成金包覆铁纳米颗粒。利用透射电子显微镜 (TEM)、化学分析电子能谱 (ESCA)、紫外可见光谱 (UV-Vis) 和 X 射线衍射 (XRD) 对所得纳米粒子进行了表征。还检查了纳米粒子的表面电荷和磁性。 Fe3O4纳米颗粒的形态为面心立方，平均直径为9.5 nm，金在Fe3O4颗粒表面的初始还原呈现出均匀的Fe3O4-Au纳米颗粒，平均直径为12.5 nm。在 300 K 下，未涂覆和金涂覆的 Fe3O4 纳米粒子的饱和磁化强度值分别为 30 和 4.5 emu/g。通过吸收光谱变化测量基于共价键相互作用的硼酸封端的配体壳和果糖之间的结合事件的进展。还在不同的大肠杆菌浓度下进行了免疫磁性分离，以评估所得纳米颗粒的捕获效率。免疫磁性分离百分比在 52.1% 和 21.9% 之间变化，具体取决于初始细菌计数。
The production of monodispersed magnetic nanoparticles with appropriate surface modification has attracted increasing attention in biomedical applications including drug delivery, separation, and purification of biomolecules from the matrices. In the present study, we report rapid and room temperature reaction synthesis of gold-coated iron nanoparticles in aqueous solution using the borohydride reduction of HAuCl4 under sonication for the first time. The resulting nanoparticles were characterized with transmission electron microscopy (TEM), electron spectroscopy for chemical analysis (ESCA), ultraviolet visible spectroscopy (UV-Vis), and X-ray diffraction (XRD). Surface charges and magnetic properties of the nanoparticles were also examined. The pattern of Fe3O4 nanoparticles is face centered cubic with an average diameter of 9.5 nm and the initial reduction of gold on the surface of Fe3O4 particles exhibits uniform Fe3O4-Au nanoparticles with an average diameter of 12.5 nm. The saturation magnetization values for the uncoated and gold-coated Fe3O4 nanoparticles were found to be 30 and 4.5 emu/g, respectively, at 300 K. The progression of binding events between boronic acid terminated ligand shell and fructose based on the covalent bonding interaction was measured by absorbance spectral changes. Immunomagnetic separation was also performed at different E. coli concentration to evaluate capturing efficiency of resulting nanoparticles. Immunomagnetic separation percentages were varied in a range of 52.1 and 21.9% depend on the initial bacteria counts.