Fgf8 drives myogenic progression of a novel lateral fast muscle fibre population in zebrafish
Fgf8 drives myogenic progression of a novel lateral fast muscle fibre population in zebrafish
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DOI：
10.1242/dev.01958
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发表时间：
2005-10-01
期刊：
影响因子：
4.6
通讯作者：
Hughes, SM
中科院分区：
文献类型：
--
作者：
Groves, JA;Hammond, CL;Hughes, SM
成纤维细胞生长因子（Fgfs）长期以来一直参与调节脊椎动物骨骼肌分化，但其在体内的确切作用仍不清楚。在这里，我们表明，FGF 8信号在体节是必需的肌样蛋白的表达和终末分化的一个子集的快肌细胞在斑马鱼侧体节。在Fgf 8的情况下，侧体节细胞瞬时表达myf 5，但不能使肌肉，并保持在一个dermomyotome-like状态，其特征在于pax 3和meox表达。缓慢的肌纤维形成，并开始正常迁移的情况下Fgf 8，但未能穿越扩展的未分化的侧体节。内侧快肌纤维的Fgf 8非依赖性残余群体不是刺猬依赖性的。然而，Fgf 8独立的内侧快肌前体缺乏floatinghead突变体，这表明他们需要另一个腹侧中线衍生的信号。我们的结论是FGF 8驱动的早期体节内的侧肌前体细胞的特定群体的终末分化。
Fibroblast growth factors (Fgfs) have long been implicated in regulating vertebrate skeletal muscle differentiation, but their precise role(s) in vivo remain unclear. Here, we show that Fgf8 signalling in the somite is required for myod expression and terminal differentiation of a subset of fast muscle cells in the zebrafish lateral somite. In the absence of Fgf8, lateral somite cells transiently express myf5 but fail to make muscle and remain in a dermomyotome-like state characterised by pax3 and meox expression. Slow muscle fibres form and commence normal migration in the absence of Fgf8, but fail to traverse the expanded undifferentiated lateral somite. The Fgf8-independent residual population of medial fast muscle fibres is not Hedgehog dependent. However, Fgf8-independent medial fast muscle precursors are lacking in floatinghead mutants, suggesting that they require another ventral midline-derived signal. We conclude that Fgf8 drives terminal differentiation of a specific population of lateral muscle precursor cells within the early somite.