Comparative genetic analysis of the antimicrobial susceptibilities and virulence of hypermucoviscous and non-hypermucoviscous ESBL-producing Klebsiella pneumoniae in Japan.
Comparative genetic analysis of the antimicrobial susceptibilities and virulence of hypermucoviscous and non-hypermucoviscous ESBL-producing Klebsiella pneumoniae in Japan.
复制标题
日本高粘液粘性和非高粘液性产 ESBL 肺炎克雷伯菌的抗菌敏感性和毒力的比较遗传分析。
DOI：
10.1016/j.jmii.2022.08.010
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Masato Fujisawa
中科院分区：
文献类型：
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作者：
Hiroshi Tanimoto;Katsumi Shigemura;Kayo Osawa;Mitsuki Kado;Reo Onishi;Shiuh-Bin Fang;Shian-Ying Sung;Takayuki Miyara;Masato Fujisawa
背景高粘滞性肺炎克雷伯菌（HMV）产生大量荚膜多糖，导致高死亡率。由于产超广谱β-内酰胺酶（ESBL）的HMVK。在日本，HMV和非HMV产ESBL的肺炎克雷伯氏菌的抗菌药物敏感性和遗传特征有所增加。方法我们调查了291株产ESBL的克雷伯氏菌。在2012年至2018年期间收集的肺炎中，鉴定了54株HMV菌株，并选择了53株可比较的非HMV菌株。用PCR扩增方法进行ESBL基因检测、质粒复制子分型和毒力基因检测。肺炎菌株具有duge（98.1%）、wabG（96.3%）、rmpA（94.4%）、iucA（79.6%）、fimH（70.4%）、iroB（70.4%）和peg-344（70.4%）。在非HMV株系中，这些基因的频率较低（uge20.8%，wabG83.0%，rmpA7.5%，iucA3.8%，fimH9.4%，iroB5.7%，peg-3441.9%）。HMV以K2荚膜型最常见（40.7%）。HMV菌株对头孢吡肟（p= 0.001）和哌拉西林/他唑巴坦（p= 0.005）的耐药性高于非HMV菌株。HMV和非HMV菌株ESBL基因型均以CTX-M-15型为主（75.9%，60.4%），质粒复制子型以IncFII型为主（52.1%）。最常见的胶囊类型为K2。CTX-M-15是日本HMV和非HMV毒株中最常见的ESBL基因类型。结论：CTX-M-15 β-K在大肠杆菌中的传播可能与FIB质粒有关。肺炎菌株
BackgroundHypermucoviscous (HMV)Klebsiella pneumoniaeproduces large amounts of capsular polysaccharides, leading to high mortality. Since extended spectrum beta-lactamase (ESBL)-producing HMVK. pneumoniaestrains have increased in Japan, we investigated and compared the antimicrobial susceptibilities and genetic characteristics of HMV and non-HMV ESBL-producingK. pneumoniae.MethodsWe investigated 291 ESBL-producingK. pneumoniaecollected between 2012 and 2018, and in them 54 HMV strains were identified and comparable 53 non-HMV strains were selected. Then, ESBL gene detection, plasmid replicon typing, and virulence gene detection were done by PCR amplification.ResultsAlmost all of the HMVK. pneumoniaestrains possesseduge(98.1%),wabG(96.3%),rmpA(94.4%),iucA(79.6%),fimH(70.4%),iroB(70.4%), andpeg-344(70.4%). These genes were found less frequently in non-HMV strains (uge20.8%,wabG83.0%,rmpA7.5%,iucA3.8%,fimH9.4%,iroB5.7%, andpeg-3441.9%). K2 capsule type (40.7%) was most common in HMV strains. HMV strains showed higher resistance to cefepime (p= 0.001) and piperacillin/tazobactam (p= 0.005) than non-HMV strains. CTX-M-15 (75.9%, 60.4%) was the dominant ESBL type in both HMV and non-HMV strains, and the most common plasmid replicon type was IncFII (52.1%) in CTX-M-15-producing strains.ConclusionsWe found that HMV strains had more virulence genes and showed higher resistance to antibiotics than non-HMV strains. The most common capsule type was K2. CTX-M-15 was the most common type of ESBL gene in both HMV and non-HMV strains in Japan. The FII plasmid might be related to the spread of CTX-M-15 amongK. pneumoniaestrains.