Environmentally driven evolution of Rubisco and improved photosynthesis and growth within the C3 genus Limonium (Plumbaginaceae)
Environmentally driven evolution of Rubisco and improved photosynthesis and growth within the C3 genus Limonium (Plumbaginaceae)
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DOI：
10.1111/nph.12858
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发表时间：
2014-08-01
期刊：
影响因子：
9.4
通讯作者：
Conesa, Miquel A.
中科院分区：
文献类型：
--
作者：
Galmes, Jeroni;Andralojc, P. John;Conesa, Miquel A.
大多数生态系统的碳同化需要核酮糖-1，5-二磷酸羧化酶/加氧酶（Rubisco）。它的动力学参数很可能已经演变与细胞内CO2的可用性平行，其结果是，更快的形式的Rubisco发生在物种与CO2-concentrationmechanism.The Rubisco催化性能进行了测定和评价地中海补血草属物种的生长和碳同化能力，密切相关的物种之间的显著动力学差异取决于功能重要残基309和309处的两个氨基酸取代。Rubisco大亚基中的328个。在干旱期间面临最大CO2限制的物种的Rubisco对CO2具有相对较高的亲和力（低Michaelis-Menten常数CO2（K-c）），但最大羧化速率（k（cat）（c））较低，而在类似条件下保持较高CO2浓度的物种则相反。Rubisco的动力学特征与光合速率在充分供水和干旱胁迫植物。此外，干旱介导的植物生物量积累的减少是一贯较低的物种具有较高的Rubisco羧化酶催化效率（k（cat）（c）/K-c）。本研究是第一次演示的Rubisco适应在物种多样化密切相关的C-3植物，揭示了Rubisco分子进化和生物量积累的密切相关的物种受到不利的条件之间的直接关系。
Carbon assimilation by most ecosystems requires ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Its kinetic parameters are likely to have evolved in parallel with intracellular CO2 availability, with the result that faster forms of Rubisco occur in species with CO2-concentrating mechanisms.The Rubisco catalytic properties were determined and evaluated in relation to growth and carbon assimilation capacity in Mediterranean Limonium species, inhabiting severe stress environments.Significant kinetic differences between closely related species depended on two amino acid substitutions at functionally important residues 309 and 328 within the Rubisco large subunit. The Rubisco of species facing the largest CO2 restrictions during drought had relatively high affinity for CO2 (low Michaelis-Menten constant for CO2 (K-c)) but low maximum rates of carboxylation (k(cat)(c)), while the opposite was found for species that maintained higher CO2 concentrations under similar conditions. Rubisco kinetic characteristics were correlated with photosynthetic rate in both well-watered and drought-stressed plants. Moreover, the drought-mediated decrease in plant biomass accumulation was consistently lower in species with higher Rubisco carboxylase catalytic efficiency (k(cat)(c)/K-c).The present study is the first demonstration of Rubisco adaptation during species diversification within closely related C-3 plants, revealing a direct relationship between Rubisco molecular evolution and the biomass accumulation of closely related species subjected to unfavourable conditions.