Activation of the rostral pontine reticular formation increases the spinal glycine level and inhibits bladder contraction in rats.
Activation of the rostral pontine reticular formation increases the spinal glycine level and inhibits bladder contraction in rats.
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DOI：
10.1097/01.ju.0000154646.11570.65
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发表时间：
2005-05
期刊：
影响因子：
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通讯作者：
S. Nishijima;K. Sugaya;M. Miyazato;S. Shimabukuro;M. Morozumi;Y. Ogawa
中科院分区：
文献类型：
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作者：
S. Nishijima;K. Sugaya;M. Miyazato;S. Shimabukuro;M. Morozumi;Y. Ogawa
目的通过对大鼠腰骶髓中氨基酸含量的分析，探讨卡巴胆碱、黄伏沙酯和丙哌维林刺激脑桥头端网状结构（RPRF）抑制膀胱活动的机制。材料与方法：82只雌性大鼠用乌拉坦麻醉。在等容条件下，将生理盐水、卡巴胆碱、flavoxate或丙哌维林注射到RPRF中或静脉内。检查膀胱活动和腰骶髓氨基酸水平的变化。结果RPRF内注射卡巴胆碱或黄酮哌酯（各0.3 μ M）可抑制膀胱收缩，但注射生理盐水或丙哌维林后无变化。静脉注射黄伏酯或丙哌维林（各0.1 ~ 10 mg/kg）可抑制膀胱收缩。氨基酸分析表明，注射卡巴胆碱到RPRF增加谷氨酸和甘氨酸水平的腰骶髓，而注射flavoxate到RPRF或静脉注射引起甘氨酸腰骶髓增加。丙哌维林注射到RPRF或静脉注射不影响腰骶髓氨基酸水平。结论RPRF在抑制膀胱收缩中起重要作用，氨甲酰胆碱或黄酮哌酯可通过激活RPRF下行神经元，抑制膀胱收缩。
PURPOSE We examined the mechanism involved in the inhibition of bladder activity in rats by stimulating the rostral pontine reticular formation (RPRF) using carbachol, flavoxate and propiverine, and by analysis of amino acid levels in the lumbosacral cord. MATERIALS AND METHODS A total of 82 female rats were anesthetized with urethane. Under isovolumetric conditions physiological saline, carbachol, flavoxate or propiverine was injected into the RPRF or intravenously. Changes in bladder activity and amino acid levels in the lumbosacral cord were examined. RESULTS Injection of carbachol or flavoxate (0.3 microM each) into the RPRF abolished bladder contraction but there was no change after injection of physiological saline or propiverine. Intravenous injection of flavoxate or propiverine (0.1 to 10 mg/kg each) inhibited bladder contraction. Amino acid analysis revealed that injection of carbachol into the RPRF increased glutamate and glycine levels in the lumbosacral cord, while injection of flavoxate into the RPRF or intravenously caused an increase in glycine the lumbosacral cord. Injection of propiverine into the RPRF or intravenously did not influence lumbosacral cord amino acid levels. CONCLUSIONS These results suggest that the RPRF has an important role in the inhibition of bladder contraction and carbachol or flavoxate can activate descending RPRF neurons and inhibit bladder contraction via spinal glycinergic neurons.