Quinolone resistance in Escherichia coli from Accra, Ghana
Quinolone resistance in Escherichia coli from Accra, Ghana
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DOI：
10.1186/1471-2180-11-44
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发表时间：
2011-02-27
期刊：
影响因子：
4.2
通讯作者：
Okeke, Iruka N.
中科院分区：
文献类型：
--
作者：
Namboodiri, Sreela S.;Opintan, Japheth A.;Okeke, Iruka N.
背景资料：抗菌药物耐药性的文献报道不足，大肠埃希氏菌可作为指示生物来研究社区中的耐药性。我们试图确定对广谱抗菌药物的耐药性的流行率，特别关注最近在非洲部分地区（包括加纳）引入的喹诺酮类药物。评价的大肠杆菌分离株是耐萘啶酸的。2006年和2007年的13株（52%）和2008年的10株（66.7%）也对环丙沙星耐药。除一株外，所有喹诺酮类耐药菌株均对三种或三种以上其他抗菌药物耐药。对喹诺酮类药物耐药决定基因gyrA和parC进行测序，发现28株耐喹诺酮类药物的大肠埃希菌中，gyrA基因和parC基因编码喹诺酮类药物的靶基因。大肠杆菌gyrA基因产物的83位有一个替换，其中20个分离株有其他gyrA和/或parC替换。在12株分离株中检测到水平获得性喹诺酮类耐药基因qnrB 1、qnrB 2、qnrS 1或qepA。尽管E.大肠埃希菌15株，耐喹诺酮类药物15株。coli属于复合体10序列类型。其中5株携带qnrS 1等位基因。大肠杆菌通常存在于阿克拉居民的粪便植物群中。分离物通过多种机制进化出抗性，并且属于非常少的谱系，表明克隆扩张。遏制战略，以限制喹诺酮类耐药大肠杆菌的传播。大肠杆菌需要部署，以保持喹诺酮类药物的有效性，并促进其替代品的使用。
Background: Antimicrobial resistance is under-documented and commensal Escherichia coli can be used as indicator organisms to study the resistance in the community. We sought to determine the prevalence of resistance to broad-spectrum antimicrobials with particular focus on the quinolones, which have recently been introduced in parts of Africa, including Ghana.Results: Forty (13.7%) of 293 E. coli isolates evaluated were nalidixic acid-resistant. Thirteen (52%) of 2006 and 2007 isolates and 10 (66.7%) of 2008 isolates were also resistant to ciprofloxacin. All but one of the quinolone-resistant isolates were resistant to three or more other antimicrobial classes. Sequencing the quinolone-resistance determining regions of gyrA and parC, which encode quinolone targets, revealed that 28 quinolone-resistant E. coli harboured a substitution at position 83 of the gyrA gene product and 20 of these isolates had other gyrA and/or parC substitutions. Horizontally-acquired quinolone-resistance genes qnrB1, qnrB2, qnrS1 or qepA were detected in 12 of the isolates. In spite of considerable overall diversity among E. coli from Ghana, as evaluated by multilocus sequence typing, 15 quinolone-resistant E. coli belonged to sequence type complex 10. Five of these isolates carried qnrS1 alleles.Conclusions: Quinolone-resistant E. coli are commonly present in the faecal flora of Accra residents. The isolates have evolved resistance through multiple mechanisms and belong to very few lineages, suggesting clonal expansion. Containment strategies to limit the spread of quinolone-resistant E. coli need to be deployed to conserve quinolone effectiveness and promote alternatives to their use.