Biologic and molecular characterization of two newly isolated ras-containing murine leukemia viruses.
Biologic and molecular characterization of two newly isolated ras-containing murine leukemia viruses.
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两种新分离的含 ras 的鼠白血病病毒的生物学和分子特征。
DOI：
10.1128/jvi.61.7.2109-2119.1987
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发表时间：
1987
影响因子：
5.4
通讯作者：
Hartley,JW
中科院分区：
文献类型：
--
作者：
Fredrickson,TN;O'Neill,RR;Rutledge,RA;Theodore,TS;Martin,MA;Ruscetti,SK;Austin,JB;Hartley,JW
从 (NFS X NS.C58v-1) F1 小鼠中回收鼠肉瘤病毒 (MSV)，该小鼠在接种水貂细胞病灶诱导鼠白血病病毒 (MuLV) 6 个月后出现脾肉瘤和红白血病，该病毒从感染野生小鼠亲嗜性 MuLV 的 NFS 小鼠中分离出来。 MSV，命名为 NS.C58 MSV-1，在小鼠和大鼠成纤维细胞中诱导转化灶，接种 2 周或更小的不同品系的小鼠会导致红白血病以及脾脏、淋巴结和大脑中的肉瘤病变。 MSV 原病毒是从转化的非生产大鼠细胞制备的基因组文库中分子克隆的。 8.8 千碱基的原病毒 DNA 包含一个 1.0 千碱基的 p21 ras 编码片段，该片段取代了 MuLV 的大部分 gp70 编码部分，很可能是内源性 C58v-1 生态病毒。 ras癌基因通过杂交、p21蛋白的表达和核苷酸序列与v-Ha-ras密切相关。它与 v-bas 的序列几乎相同，v-bas 是先前描述的唯一转导、激活的小鼠 c-ras。在 p21 编码区的第 12 位，精氨酸取代了 c-ras 中天然存在的甘氨酸。描述了第二种MSV分离株，其与NS.C58 MSV-1相似，除了在包含ras的插入片段的非编码区中存在100至200个碱基对缺失。
A murine sarcoma virus (MSV) was recovered from an (NFS X NS.C58v-1) F1 mouse which developed splenic sarcoma and erythroleukemia 6 months after inoculation with a mink cell focus-inducing murine leukemia virus (MuLV) isolated from an NFS mouse infected with a wild mouse ecotropic MuLV. The MSV, designated NS.C58 MSV-1, induced foci of transformation in mouse and rat fibroblasts, and inoculation of mice of various strains 2 weeks of age or younger resulted in erythroleukemia and sarcomatous lesions in spleen, lymph node, and brain. The MSV provirus was molecularly cloned from a genomic library prepared from transformed non-producer rat cells. The 8.8-kilobase proviral DNA contained a 1.0-kilobase p21 ras coding segment which replaced most of the gp70-encoding portion of an MuLV, most likely the endogenous C58v-1 ecotropic virus. The ras oncogene is closely related to v-Ha-ras by hybridization, expression of p21 protein, and nucleotide sequence. It is nearly identical in sequence to v-bas, the only previously described transduced, activated mouse c-ras. At position 12 in the p21 coding region, arginine is substituted for the naturally occurring glycine present in c-ras. A second MSV isolate is described which is similar to NS.C58 MSV-1 except for a 100- to 200-base-pair deletion in the noncoding region of the ras-containing insert.