STRUCTURE OF LATE ADENOVIRUS-2 HETEROGENEOUS NUCLEAR-RNA
STRUCTURE OF LATE ADENOVIRUS-2 HETEROGENEOUS NUCLEAR-RNA
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DOI：
10.1016/0022-2836(79)90468-6
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发表时间：
1979-01-01
影响因子：
5.6
通讯作者：
SHARP, PA
中科院分区：
文献类型：
--
作者：
BERGET, SM;SHARP, PA
晚期腺病毒2 mRNA由长度约20，000个核苷酸的单个转录单位加工而成。所有细胞质RNA都有多个前导序列剪接到它们的5“末端;最常见的形式是三重前导序列集。与5“近端前导序列互补的序列位于位置17附近，位置17是该转录单位上的起始位点。晚期腺病毒2感染的[人宫颈癌HeLa]细胞中稳态核RNA的结构通过核RNA和限制性内切酶切割片段的杂交体的EM、用单链特异性核酸酶消化类似杂交体并通过琼脂糖凝胶电泳解析受保护的DNA链来分析。虽然所有细胞质mRNA在切除适当的间插序列后都有3个或更多个剪接在一起的前导片段，但长的核RNA含有这些间插序列中的一些或全部。长的完全共线的核RNA在基因组上的位置17处具有5“末端。所有其他具有1或2个间插片段切除的核RNA形式具有与5“近端剪接必须在间插序列的第2个内部片段被切除之前发生的命题一致的结构。长的核病毒RNA通常具有独特的3“末端，其定位在与细胞质mRNA的3”末端相同的位置。稳态核晚期腺病毒2 RNA的结构与这些分子是来自长得多的初始转录物的mRNA的分子内加工中的中间体一致。
Late adenovirus 2 mRNA are processed from a single transcriptional unit some 20,000 nucleotides in length. All cytoplasmic RNA have multiple leaders spliced to their 5'' termini; the most common form is a tripartite leader set. Sequences complementary to the 5'' proximal leader lie adjacent to position 17, the site for initiation on this transcriptional unit. The structure of steady-state nuclear RNA in late adenovirus 2-infected [human cervical carcinoma HeLa] cells was analyzed by EM of hybrids of nuclear RNA and restriction endonuclease cleavage fragments, and digestion of similar hybrids with single-strand-specific nucleases and resolving the protected DNA strands by electrophoresis in agarose gels. While all cytoplasmic mRNA have 3 or more leader segments spliced together following excision of appropriate intervening sequences, long nuclear RNA contain some or all of these intervening sequences. Long completely colinear nuclear RNA have 5'' termini at position 17 on the genome. All other nuclear RNA forms with 1 or 2 intervening segments excised have a structure consistent with the proposition that the 5'' proximal splice must occur before the 2nd internal segment of intervening sequences can be excised. Long nuclear viral RNA typically have unique 3'' termini, which map in the same position as the 3'' termini of cytoplasmic mRNA. The structure of steady-state nuclear late adenovirus 2 RNA is consistent with these molecules being intermediates in the intramolecular processing of mRNA from a much longer initial transcript.