Cell proliferation, apoptosis, and B- and T-lymphocytes in Peyer's patches of the ileum, in thymus and in lymph nodes of preterm calves, and in full-term calves at birth and on day 5 of life
Cell proliferation, apoptosis, and B- and T-lymphocytes in Peyer's patches of the ileum, in thymus and in lymph nodes of preterm calves, and in full-term calves at birth and on day 5 of life
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DOI：
10.3168/jds.s0022-0302(03)73934-4
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发表时间：
2003-10-01
影响因子：
3.5
通讯作者：
Blum, JW
中科院分区：
文献类型：
--
作者：
David, CW;Norrman, J;Blum, JW
派尔集合淋巴结、胸腺和淋巴结包含大多数淋巴细胞。我们研究了未进食的早产犊牛（GrP组；在给母牛注射前列腺素F - 2α和糖皮质激素后，比预期正常分娩时间提前13天出生）以及正常足月出生的犊牛（GrF组）在出生时以及出生后喂食初乳4天（GrC组）第5天的回肠派尔集合淋巴结、胸腺以及肠系膜和肩胛前淋巴结（LM和LP）中B淋巴细胞和T淋巴细胞的增殖率、凋亡率以及数量。分别采用免疫组织化学方法结合5 - 溴 - 2'-脱氧尿苷掺入法或末端脱氧核苷酸转移酶介导的X - dUTP缺口末端标记法来评估细胞增殖率和凋亡率。分别用针对CD3和CD79的单克隆抗体测定T淋巴细胞和B淋巴细胞的数量。与GrP组相比，GrF组的LM和LP中增殖细胞和凋亡细胞数量更多，LM和LP的副皮质区和滤泡中的B淋巴细胞数量更多，胸腺皮质和髓质中的增殖细胞数量更多。在胸腺皮质和髓质中，GrC组的增殖细胞数量比GrF组多。GrC组派尔集合淋巴结所有部位的凋亡率通常比GrF组低，并且在滤泡内区域从GrP组到GrF组增殖率增加，在所有组织中从GrF组到GrC组增殖率增加。GrF组派尔集合淋巴结中的T淋巴细胞数量比GrP组多，但GrC组比GrF组少，除了圆顶区。尽管增殖率高且凋亡率低，但派尔集合淋巴结中的B淋巴细胞数量没有变化，这表明它们在生命的最初几天离开派尔集合淋巴结。总之，回肠派尔集合淋巴结、胸腺以及LM和LP中B淋巴细胞和T淋巴细胞的增殖率、凋亡率以及数量呈现出不同的发育变化，并受喂食的影响。
Peyer's patches, thymus, and lymph nodes contain the majority of lymphocytes. We have studied proliferation rates, apoptosis rates, and numbers of B- and T-lymphocytes in Peyer's patches in ileum, thymus, and mesenterial and prescapular lymph nodes (LM and LP) in unfed preterm calves (GrP; born 13 d before expected normal term after dams were injected with prostaglandin F-2alpha and glucocorticoids) and normal-term calves (GrF) immediately after birth and on d 5 of life after feeding colostrum for 4 d (GrC). Immunohistochemical methods in conjunction with incorporation of 5- bromo-2'-deoxyuridine or terminal deoxynucleotidyl transferase-mediated X-dUTP nick end labeling were used to evaluate cell proliferation rates and apoptosis rates, respectively. The number of T- and B- lymphocytes was determined with monoclonal antibodies directed against CD3 and CD79, respectively. In GrF compared with GrP, there were higher numbers of proliferating and apoptotic cells in LM and LP, of B- lymphocytes in paracortex and follicles of LM and LP, and of proliferating cells in cortex and medulla of thymus. In thymus cortex and medulla, numbers of proliferating cells were higher in GrC than in GrF. Apoptotic rates were generally smaller at all sites of Peyer's patches in GrC than in GrF, and proliferation rates increased from GrP to GrF in intrafollicular areas and from GrF to GrC in all tissues. Numbers of T- lymphocytes in Peyer's patches were higher in GrF than in GrP, but lower in GrC than in GrF, except in the domes. Numbers of B- lymphocytes did not change in Peyer's patches despite high proliferation and low apoptotic rates, suggesting that they leave Peyer's patches during the first days of life. In conclusion, proliferation and apoptosis rates and numbers of B- and T- lymphocytes in Peyer's patches in ileum, thymus, and LM and LP exhibited different developmental changes and were affected by feeding.