VISUALIZATION OF STEROID-3-BETA-OL-DEHYDROGENASE ACTIVITY IN TISSUES OF INTACT AND HYPOPHYSECTOMIZED RATS
VISUALIZATION OF STEROID-3-BETA-OL-DEHYDROGENASE ACTIVITY IN TISSUES OF INTACT AND HYPOPHYSECTOMIZED RATS
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DOI：
10.1210/endo-65-6-932
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发表时间：
1959-01-01
期刊：
影响因子：
4.8
通讯作者：
RUBIN, BL
中科院分区：
文献类型：
--
作者：
LEVY, H;DEANE, HW;RUBIN, BL
当切片在含有底物、丙二醇、DPN、烟酰胺和四唑盐（Nitro-BT）的磷酸盐缓冲液（pH 7.1-7.4）中孵育时，在大鼠肾上腺、卵巢和睾丸以及一些输卵管上皮中的所有类型的类固醇产生细胞中均可证明脱氢表雄酮（一种3[β]-羟基类固醇）脱氢。在肝脏或肾脏中对该底物无明显活性。冷丙酮处理后，活性受到严重抑制的卵泡内膜，但不是在其他网站。类固醇-3 [beta]-醇脱氢酶似乎比广泛分布的DPN依赖性脱氢酶乳酸脱氢酶更不稳定。垂体切除后，大鼠卵巢和肾上腺皮质中的酶活性仍然很明显，仅在严重萎缩的残留类固醇产生细胞（卵巢间质细胞，肾上腺皮质的内区）中，1-2个月后酶活性下降。促肾上腺皮质激素治疗恢复长期垂体切除大鼠肾上腺皮质活动。17-羟基类固醇的一些脱氢发生在相同的组织中，也发生在肝脏和肾脏中。
Dehydrogenation of dehydroepiandrosterone, a 3[beta]-hydroxysteroid, is demonstrable in all types of steroid-producing cells in the rat adrenal gland, ovary and testis, as well as in some of the oviduct epithelium, when sections are incubated in a phosphate buffer (pH 7.1-7.4) solution containing substrate, propylene glycol, DPN, nicotinamide and the tetrazolium salt, Nitro-BT. No activity toward this substrate is evident in liver or kidney. After cold acetone treatment, activity is severely inhibited in the follicular theca interna but not in the other sites. Steroid-3[beta]-ol dehydrogenase appears more labile than the widely-distributed DPN-dependent dehydrogenase, lactic dehydrogenase. Enzymatic activity remains conspicuous in rat ovary and adrenal cortex after hypophysectomy, declining after 1-2 months only in those residual steroid-producing cells that have become severely atrophied (ovarian interstitial cells, inner zones of the adrenal cortex). Treatment with ACTH restores adrenocortical activity in long-term hypophysectomized rats. Some dehydrogenation of 17-hydroxysteroids occurs in the same tissues and also in liver and kidney.